No, it is not sulfenic acid or so, I had that in differend structure. It is L shaped from S, and linear if I put SCX mutaion (another 1.4A way a nice blob). My resolution is 1.3 A.
On Tue, May 12, 2015 at 4:18 PM, Artem Evdokimov <artem.evdoki...@gmail.com> wrote: > Looks like a wrong shape for tetrahedral acid > ... > On May 12, 2015 4:07 PM, "Boaz Shaanan" <bshaa...@bgu.ac.il> wrote: > >> Hi, >> >> >> As I've just written to David, maybe it's sulfenic acid (result of >> radiation damage?). I've had those in some cases. See attached scheme for >> the chemistry and other possibilities for Cys modification by oxidation. >> >> Boaz >> >> >> >> >> >> >> >> >> >> >> >> *Boaz Shaanan, Ph.D. Dept. of >> Life Sciences Ben-Gurion University of >> the Negev Beer-Sheva >> 84105 >> Israel >> E-mail: >> bshaa...@bgu.ac.il <bshaa...@bgu.ac.il> Phone: 972-8-647-2220 Skype: >> boaz.shaanan Fax: 972-8-647-2992 or 972-8-646-1710 * >> >> >> >> >> >> ------------------------------ >> *From:* CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] on behalf of Bio >> Physics [biophysics.w...@gmail.com] >> *Sent:* Tuesday, May 12, 2015 11:57 PM >> *To:* CCP4BB@JISCMAIL.AC.UK >> *Subject:* Re: [ccp4bb] CYS mod >> >> >> Hi All, >> >> I have same type of modified CYS !!! >> No BME. >> >> On Tue, May 12, 2015 at 2:59 PM, David Schuller <schul...@cornell.edu> >> wrote: >> >>> So far almost everyone is suggesting BME. >>> >>> I didn't do the purification & crystallization myself, but I am told: >>> >>> Buffer was HEPES >>> cryo was ethylene glycol, glycerol and DMSO. >>> Some MgSO4 and KPO4 >>> No BME, but maybe some DTT. >>> >>> Purified from a proteobacterium. >>> >>> >>> >>> >>> On 05/12/15 15:20, Dyda wrote: >>> >>>> What is in buffer? Perhaps DTT and cacodylate? >>>> >>>> Fred >>>> >>>> >>>> [32m******************************************************************************* >>>> Fred Dyda, Ph.D. Phone:301-402-4496 >>>> Laboratory of Molecular Biology Fax: 301-496-0201 >>>> DHHS/NIH/NIDDK e-mail:fred.d...@nih.gov >>>> Bldg. 5. Room 303 >>>> Bethesda, MD 20892-0560 URGENT message e-mail: >>>> 2022476...@mms.att.net >>>> Google maps coords: 39.000597, -77.102102 >>>> http://www2.niddk.nih.gov/NIDDKLabs/IntramuralFaculty/DydaFred >>>> ******************************************************************************* >>>> [m >>>> >>> >>> >>> -- >>> ======================================================================= >>> All Things Serve the Beam >>> ======================================================================= >>> David J. Schuller >>> modern man in a post-modern world >>> MacCHESS, Cornell University >>> schul...@cornell.edu >>> >> >>
