Is there any reason to be S-S-CH3 modification of a specific CYS! Others CYSs are fine.
On Tue, May 12, 2015 at 5:27 PM, Finke Aaron (PSI) <aaron.fi...@psi.ch> wrote: > What are the bond lengths- or, rather, the centers from blob to blob? > Looks a bit short to be S-O, could be S-S-CH3? > > Cheers, > Aaron > ------------------------------------------ > Dr. Aaron Finke > Postdoctoral Fellow > Swiss Light Source > WSLA/217 > CH-5232 Villigen-PSI > phone: +41 56 310 5652 > e-mail: aaron.fi...@psi.ch > > On May 13, 2015, at 0:15, Bio Physics <biophysics.w...@gmail.com> wrote: > > No, it is not sulfenic acid or so, I had that in differend structure. It > is L shaped from S, and linear if I put SCX mutaion (another 1.4A way a > nice blob). My resolution is 1.3 A. > > > > On Tue, May 12, 2015 at 4:18 PM, Artem Evdokimov < > artem.evdoki...@gmail.com> wrote: > >> Looks like a wrong shape for tetrahedral acid >> ... >> On May 12, 2015 4:07 PM, "Boaz Shaanan" <bshaa...@bgu.ac.il> wrote: >> >>> Hi, >>> >>> >>> As I've just written to David, maybe it's sulfenic acid (result of >>> radiation damage?). I've had those in some cases. See attached scheme for >>> the chemistry and other possibilities for Cys modification by oxidation. >>> >>> Boaz >>> >>> >>> >>> >>> >>> >>> >>> >>> >>> >>> >>> *Boaz Shaanan, Ph.D. Dept. of >>> Life Sciences Ben-Gurion University of >>> the Negev Beer-Sheva >>> 84105 >>> Israel >>> E-mail: >>> bshaa...@bgu.ac.il <bshaa...@bgu.ac.il> Phone: 972-8-647-2220 Skype: >>> boaz.shaanan Fax: 972-8-647-2992 or 972-8-646-1710 * >>> >>> >>> >>> >>> >>> ------------------------------ >>> *From:* CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] on behalf of Bio >>> Physics [biophysics.w...@gmail.com] >>> *Sent:* Tuesday, May 12, 2015 11:57 PM >>> *To:* CCP4BB@JISCMAIL.AC.UK >>> *Subject:* Re: [ccp4bb] CYS mod >>> >>> >>> Hi All, >>> >>> I have same type of modified CYS !!! >>> No BME. >>> >>> On Tue, May 12, 2015 at 2:59 PM, David Schuller <schul...@cornell.edu> >>> wrote: >>> >>>> So far almost everyone is suggesting BME. >>>> >>>> I didn't do the purification & crystallization myself, but I am told: >>>> >>>> Buffer was HEPES >>>> cryo was ethylene glycol, glycerol and DMSO. >>>> Some MgSO4 and KPO4 >>>> No BME, but maybe some DTT. >>>> >>>> Purified from a proteobacterium. >>>> >>>> >>>> >>>> >>>> On 05/12/15 15:20, Dyda wrote: >>>> >>>>> What is in buffer? Perhaps DTT and cacodylate? >>>>> >>>>> Fred >>>>> >>>>> >>>>> [32m******************************************************************************* >>>>> Fred Dyda, Ph.D. Phone:301-402-4496 >>>>> Laboratory of Molecular Biology Fax: 301-496-0201 >>>>> DHHS/NIH/NIDDK e-mail:fred.d...@nih.gov >>>>> Bldg. 5. Room 303 >>>>> Bethesda, MD 20892-0560 URGENT message e-mail: >>>>> 2022476...@mms.att.net >>>>> Google maps coords: 39.000597, -77.102102 >>>>> http://www2.niddk.nih.gov/NIDDKLabs/IntramuralFaculty/DydaFred >>>>> ******************************************************************************* >>>>> [m >>>>> >>>> >>>> >>>> -- >>>> ======================================================================= >>>> All Things Serve the Beam >>>> ======================================================================= >>>> David J. Schuller >>>> modern man in a post-modern world >>>> MacCHESS, Cornell University >>>> schul...@cornell.edu >>>> >>> >>> > >
