[ccp4bb] ACA Abstract Deadline Friday March 31 for Speakers

Good morning, Just a reminder that the abstract deadline for oral presentations at the 75th American Crystallographic Association Meeting is fast approaching. The meeting will be in Lombard, IL (outside of Chicago) and is scheduled for 7/18/25-7/23/25. Lots of great sessions, workshops, and p

Re: [ccp4bb] Pipetting Robot

We use the Formulator from Formulatrix for mixing cocktails. We use it to mix 96 well deep well blocks for preparing the 1536 high-throughput screen we use in the National Crystallization Center at HWI, as well as for optimization plates. I'm not sure if any of the others can dispense into dee

[ccp4bb] Metals in Structural Biology Workshop at the SSRL/LCLS Users' Meeting at SLAC National Accelerator Laboratory

, University of Michigan & Sarah Bowman, Hauptman-Woodward Institute/University at Buffalo (organizers) 9:00 AM Organometallic Masquerade at the heart of Wood-Ljungdahl: strides toward the CO intermediate of carbon monoxide dehydrogenase - Macon Abernathy, SLAC National Accelerator Laboratory 9:3

[ccp4bb] Advancing Drug Discovery Webinar Series - Stephen Burley speaking tomorrow 6/4/2024

Hello colleagues, The next webinar in the Advancing Drug Discovery: A Webinar Series of the National Academies of Sciences, Engineering and Medicine will feature Professor Stephen K. Burley. His talk is entitled Protein Data Bank: From two epidemics & the global pandemic to mRNA vaccines & Pax

[ccp4bb] 2024 Applied Crystallization Workshop

Hi CCP4, Announcing the Applied Crystallization Workshop at the National Crystallization Center at HWI in Buffalo, NY, USA. Does your macromolecular crystallization project feel like the Wild West? Join our two-part Crystallization Workshop to learn to wrangle your crystals! What is it? A mac

[ccp4bb] 2024 ACA Meeting - Abstract and Travel Grants Deadline Friday 3/29/2024

Hi everyone, The 2024 Annual ACA Meeting will be in Denver, CO from July 7 - July 11, 2024! Program details https://www.acameeting24.com/sessionlist. The abstract submission deadline is this Friday March 29, 2024! There is a great lineup of sessions and abstracts are still being accepted – che

Re: [ccp4bb] Fragile Crystals

Hi Morgan, Some of the things that you could try with the conditions that you have already found: 1) Lower the temperature for incubation, which might slow down crystal formation and decrease potential defects as the crystals form 2) Harvest the crystals using a humidity control device like th

[ccp4bb] Metals in Structural Biology workshop at SSRL/LCLS User Meeting - tomorrow

Hi everyone, Just a reminder that the Metals in Structural Biology workshop at the SSRL/LCLS User Meeting is tomorrow Friday Sept 29. Here is a link to the agenda, which also lists zoom access if you are unable to attend in person (most talks will also be available via zoom): https://events.bi

[ccp4bb] SSRL/LCLS Users' Meeting

Hi all, There is still time to register and attend the 2023 SSRL/LCLS Users’ Meeting – we are excited that the meeting will be in person Sept 24-29! Registration link: https://events.bizzabo.com/SLAC-2023/home The Metals in Structural Biology workshop is Friday Sept 29, and will include the fo

[ccp4bb] Postdoctoral Associate Position Bowman Lab at HWI

letter describing your interest in the position and your career goals, as well as a current CV and contact information (name, phone, and email) for three references to Sarah Bowman, PhD to: sbowman at hwi.buffalo.edu LEARN MORE: HWI is a thriving site for structural biology research, with

[ccp4bb] HTX Center at HWI User Meeting Announcement

Hi CCP4BB! I am excited to announce that the National High-Throughput Crystallization (HTX) Center at HWI will be hosting a Virtual User's Meeting! The meeting is scheduled on Friday 3/31/2023 from 10am-3pm (Eastern). Register here

[ccp4bb] 2023 ACA Meeting - Abstracts due March 24

One Weird Trick (Co-chairs: Charles Bou-Nader, Jonathan Hermann) 4.2.4: Electron Tomography (Co-chairs: Lindsey Backman, Devrim Acehan) 4.2.5: Microcrystal Electron Diffraction of Proteins (MicroED) (Co-chairs: Sarah Bowman, Brent Nannenga) Cheers, Sarah Sarah EJ Bowman PhD Associate Investigator

[ccp4bb] Metals in Structural Biology Workshop Friday Sept 30 during the SSRL/LCLS Users' Meeting

ion on Metals in Structural Biology (schedule<https://events.bizzabo.com/SLAC-UsersMeeting-2022/agenda/session/897902>) – please join us. Speakers: Alison Biester (MIT) Sarah Bowman (HWI) Andy Borovik (UC Irvine) Allen Orville (Diamond Light Source) Stephen Ragsdale (U Michigan) Katie Rush (O

Re: [ccp4bb] Crystallization Robots

rystallization Center Webpage<http://www.getacrystal.org> Hauptman-Woodward Medical Research Institute 700 Ellicott Street | Buffalo, NY 14203 From: Shawn Rumrill Date: Monday, May 23, 2022 at 12:45 PM To: Edward Snell , "CCP4BB@JISCMAIL.AC.UK" Cc: Sarah Bowman Subject: Re: [ccp4bb]

[ccp4bb] ACA Abstract Deadline Today

Hi everyone, Just a reminder that the abstract deadline for the 2021 virtual American Crystallographic Association meeting is today, Sunday 5/23/21! Meeting dates July 30 – August 5, 2021. Submit an abstract: https://www.acameeting.com/ Sessions being sponsored or co-sponsored by BioMac SIG:

[ccp4bb] IUCr 'Crystallography against Corona' Session

Announcing a late breaking session at the upcoming 2021 IUCr Congress – Crystallography against Corona. This session will cover the efforts of the crystallographic community to find drug candidates for COVID-19 both in-silico and in the lab by using structural models of viral proteins. The ses

Re: [ccp4bb] Elemental Analysis

at hwi.buffalo.edu Research Webpage<https://hwi.buffalo.edu/scientist-directory/sbowman/> www.getacrystal.org<http://www.getacrystal.org> Hauptman-Woodward Medical Research Institute 700 Ellicott Street | Buffalo, NY 14203 From: CCP4 bulletin board on behalf of Sarah Bowman Reply-To

Re: [ccp4bb] Elemental Analysis

Hi Andy, We were able to use EDX fluorescence scans at a beam line to detect calcium in crystals. https://pubmed.ncbi.nlm.nih.gov/29890074/ It isn't quantitative like micro-pixe but you can see it... Eddie Snell and Elspeth Garman and team have done some further work on micro-pixe, as well –

[ccp4bb] Crystallization screening for COVID-19 research

Hello everyone, As we all work towards applying structural techniques in the scientific efforts geared towards resolving the COVID pandemic, I wanted the community to be aware that the HWI Crystallization Center is available for crystallization screening for COVID-19 related structural work (no

Re: [ccp4bb] protein protein interactions

Hi Careina, There’s a program called FRODOCK that generates predictions of how two proteins could interact: http://chaconlab.org/modeling/frodock Cheers, Sarah Sarah EJ Bowman, PhD Associate Research Scientist, Hauptman-Woodward Medical Research Institute Director, High-Throughput Crystallizat

Re: [ccp4bb] Macromolecular Crystallography workshop in South America 2020

It's been an enlightening discussion. There are a number of resources available to help the scientific community with these issues, including a new app that was described just yesterday in Nature Index: https://tinyurl.com/swu3ecn Sarah Sarah EJ Bowman, PhD Associate Research Scientist, Haup

Re: [ccp4bb] Unusual monomer-monomer interface in crystal

This conversation brings up the question of whether it would be good practice to always do a fluorescence scan of a crystal sample, whether it is known to be a metalloprotein or not... Energy dispersive X-ray fluorescence spectra (accessible at most synchrotron sources at this point) can often

Re: [ccp4bb] Sodium Ion Binding?

Hi Jacob, An additional technique to identify sodium is microPIXE, which can be used for elemental mapping. I'm not sure if the sulfur signal would be strong enough to be quantitative. A ref: https://www.cell.com/structure/pdf/S0969-2126(00)88335-5.pdf Hope that helps, Sarah Sarah EJ Bowman,

Re: [ccp4bb] Importance of temperature during initial crystallization screening

Temperature is an interesting parameter, but I agree with Janet and others that it is difficult to predict what will work best. At the High-Throughput Crystallization Screening Center, we typically run 23C, 14C, or 4C screening experiments to screen for initial crystallization conditions. We

Re: [ccp4bb] challenges in structural biology

I'd like to point out that the MAchine Recognition of Crystallization Outcomes (MARCO) makes a start to 'deep learning applied to crystallization outcomes', at least in terms of being able to classify drop images efficiently. There is obviously more work to be done to correlate these data with

Re: [ccp4bb] challenges in structural biology

Hi James, 2 things come to mind. 1) What are the tools we can use for metals in structural biology? Note, I am biased here… 2) Micro electron diffraction methods - ability to use on small crystals (which speaks to Tom’s point) and a potential bridge between cryo-EM and X-ray diffraction techn

Re: [ccp4bb] wanted: Hampton Research Glucose Isomerase Crystals

Hi Eike, We have a small amount of HR glucose isomerase crystals. Happy to send an aliquot to you – send me an email and we can connect about details! Cheers, Sarah Sarah EJ Bowman, PhD Associate Research Scientist, Hauptman-Woodward Medical Research Institute Director, High-Throughput Cryst

Re: [ccp4bb] normal-coordinate structural decomposition (NSD) server?

Hi Mike, Here is a link to a current NSD calculator for heme distortions from planarity available online from the Liptak Group in Vermont: http://mliptak.w3.uvm.edu/nsd.html This uses the method originally developed by Shelnutt. Hope that helps! Sarah Sarah EJ Bowman, PhD High-Throughput Cr