As a follow-up, we have a postdoc position open to further develop metal identification methods for crystals and in solution!
The project: structural study of biological systems that are impacted by free radical activity and metalloproteins, where the modeled metal and/or coordination state are of interest for further investigation. The work will use X-ray crystallography, UV-spectroscopy, and micro-PIXE techniques. The eventual aim involves structural studies using X-ray free-electron sources for radiation chemistry-free data. It is possible that neutron studies would also take place. The position will be based at HWI through the University at Buffalo in the Snell<https://hwi.buffalo.edu/scientist-directory/snell/> and Bowman<https://hwi.buffalo.edu/scientist-directory/sbowman/> laboratories. More details<https://hwi.buffalo.edu/about-us-old/careers/>. Happy New Year! Sarah Sarah EJ Bowman PhD Associate Research Scientist | Hauptman-Woodward Medical Research Institute Director | High-Throughput Crystallization Screening Center Research Associate Professor | Department of Biochemistry | University at Buffalo p: +1 716 898 8623 e: sbowman at hwi.buffalo.edu Research Webpage<https://hwi.buffalo.edu/scientist-directory/sbowman/> www.getacrystal.org<http://www.getacrystal.org> Hauptman-Woodward Medical Research Institute 700 Ellicott Street | Buffalo, NY 14203 From: CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK> on behalf of Sarah Bowman <sbow...@hwi.buffalo.edu> Reply-To: Sarah Bowman <sbow...@hwi.buffalo.edu> Date: Thursday, January 7, 2021 at 9:52 AM To: "CCP4BB@JISCMAIL.AC.UK" <CCP4BB@JISCMAIL.AC.UK> Subject: Re: Elemental Analysis Hi Andy, We were able to use EDX fluorescence scans at a beam line to detect calcium in crystals. https://pubmed.ncbi.nlm.nih.gov/29890074/ It isn't quantitative like micro-pixe b This sender is trusted. sophospsmartbannerend Hi Andy, We were able to use EDX fluorescence scans at a beam line to detect calcium in crystals. https://pubmed.ncbi.nlm.nih.gov/29890074/ It isn't quantitative like micro-pixe but you can see it... Eddie Snell and Elspeth Garman and team have done some further work on micro-pixe, as well – very cool stuff! https://pubmed.ncbi.nlm.nih.gov/31794207/ Cheers, Sarah Sarah EJ Bowman PhD Associate Research Scientist | Hauptman-Woodward Medical Research Institute Director | High-Throughput Crystallization Screening Center Research Associate Professor | Department of Biochemistry | University at Buffalo p: +1 716 898 8623 e: sbowman at hwi.buffalo.edu Research Webpage<https://hwi.buffalo.edu/scientist-directory/sbowman/> Crystallization Center Webpage<http://www.getacrystal.org> Hauptman-Woodward Medical Research Institute 700 Ellicott Street | Buffalo, NY 14203 From: CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK> on behalf of Andrew Lovering <a.lover...@bham.ac.uk> Reply-To: Andrew Lovering <a.lover...@bham.ac.uk> Date: Thursday, January 7, 2021 at 9:13 AM To: "CCP4BB@JISCMAIL.AC.UK" <CCP4BB@JISCMAIL.AC.UK> Subject: Elemental Analysis Dear CCP4ers We have a protein with moderate resolution (mid 2s) with obvious metal ion density holding the oligomer together. We can make an educated guess that the ions are likely to be Mg/Ca This sender is trusted. sophospsmartbannerend Dear CCP4ers We have a protein with moderate resolution (mid 2s) with obvious metal ion density holding the oligomer together. We can make an educated guess that the ions are likely to be Mg/Ca and can look at co-ordination and bond lengths…..but I have a more specific question – would it be appropriate to try and identify these at the beamline? Obviously the edges for these aren’t accessible to sit either side of the peak but perhaps something like micro-pixe would be fun; is this done routinely? https://doi.org/10.1016/j.pbiomolbio.2004.09.005 Best & thanks in advance Andy ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
