s,
implied *discarding *the most important part of the available information
(see the Why-O-Why ).
(
https://www.linkedin.com/posts/marin-van-heel-5845b422b_whyowhyarchive-activity-7149738255154946048-Oc93/?utm_source=share&utm_medium=member_desktop
).
That problem was realized soon and the mentione
Dear All,
Sayan Bhakta and I have recently posted the preprint of a review on
resolution and linearity which will appear in a book to be launched on the
16th of October 2024.
( https://doi.org/10.1201/9781003326106 ).
It is the first Cryo-EM review that I have been involved in for 25 years.
In our
e is also
an easy-to-find Youtube lecture on the issue by "marin van heel").
Cheers,
Marin
On Tue, Oct 26, 2021 at 3:58 AM Jacob Keller wrote:
> Hi All,
>
> haven't been following CCP4BB for a while, then I come back to this juicy
> Holtonian thread!
>
> Sorry for
enough to get a gist of the port of the
matter.
More than just two pennies worth (we hope),
Cheers
Marin
(PS: we have applied for the necessary patents...)
https://arxiv.org/abs/2009.03223
Google "arXiv" for "Information: to Harvest, to Have and to Hold"
by: Marin van Heel
One more remark on the camera correction issue...
The camera correction does actually matter quite a bit, especially where it
concerns the movie alignments in the early phases of processing, as Tanaka
mentioned too. What I want to point out here is that the effect of the
camera correction needs t
Dear Wout,
A suggestion from the inventor of the FRC/FSC: please read the discussions
on using non-linear refinement procedures to optimise linear metrics beyond
their defined validity range in: "Information: to Harvest, to Have and to
Hold", by Marin van Heel & Michael Schatz.
(htt
tting involved
> in the
> discussion, which is exactly the opposite of what a thread on a
> scientific
> bulletin board should be doing.
>
>
> With best wishes,
>
> Gerard.
>
> --
> On Sun, Feb 23, 2020 at 08:15:34AM -0300, Marin van Heel
s in large boxes, and a number of other
> conditions can make fixed thresholds dangerous.
>
> It would indeed be better to use a non-fixed threshold. So why am I not
> using the 1/2-bit criterion in my own work? While numerically it behaves
> well at most resolution ranges, I was not con
> reading of the paper would reveal to you this much.
>
> Regards,
> Pawel
>
> On Feb 16, 2020, at 10:38 AM, Marin van Heel
> wrote:
>
>
>
> * EXTERNAL EMAIL *
> Dear Pawel and All others
>
> This 2010 review is - unfortunately - largely base
tion related to the estimated error
> on some observed hydrogen bond length of interest, or an error on the
> estimated occupancy of a ligand or conformation or anything else that has
> structural significance?
>
>
>
> In crystallography, it isn't really (only in some
Dear Colin
Great that you mention the Rose equation and its consequences for cryo-EM!
I have actually written a paper on that topic some 40 years ago [Marin van
Heel: Detection of object in quantum-noise limited images. Ultramicroscopy
8 (1982) 331-342]. I honestly have not thought about it for
issue alone…*
*Marin van Heel, CNPEM/LNNano, Campinas, Brazil *
On Sun, Feb 16, 2020 at 6:51 PM Petrus Zwart wrote:
> Hi All,
>
> How is the 'correct' resolution estimation related to the estimated error
> on some observed hydrogen bond length of interest, or an er
Dear Pawel and All others
This 2010 review is - unfortunately - largely based on the flawed
statistics I mentioned before, namely on the a priori assumption that the
inner product of a signal vector and a noise vector are ZERO (an
orthogonality assumption). The (Frank & Al-Ali 1975) paper we
nih.gov/pubmed/30198894 which is sort of along
> similar lines of what you are hinting here.
> Pavel
>
> On Wed, Feb 12, 2020 at 3:07 PM Marin van Heel <
> 057a89ab08a1-dmarc-requ...@jiscmail.ac.uk> wrote:
>
>> Hi Tim,
>> Good to hear from you! No longer at
ary number of
> random re-orientations of such a helix, refine, and calculate the
> variation in
> position and rotation.
>
> This would reflect my understanding of resolution, much more than any
> statistical descriptor.
>
> Best regards,
> Tim
>
> On Wednesday, February
Dear Pietro
I have used many different gratings for the purpose over many years...
Ones that I found very rewarding are writeable CDs and DVDs. They often
come in a pack "protected" by a empty CD / DVD matrix hat is only plastic
with grooves and no silver (or whatever the shiny recording material
Hello Jacob
I know it was a bit of a cynical joke and I know it does hurt ... After
all my mother's Ellis Island records are dated 1923. Our last family
reunion was 15 years ago in Illinois and Missouri. My Mid-West raised uncle
was torpedoed off the coast of Cape Canaveral in 1942 transporting U
142376”
==
Cheers
Marin
--
==
Prof Dr Ir Marin van Heel
Laboratório Nacional de Nanotecnologia - LNNano
CNPEM/LNNano, Campinas, Brazil
tel: +55-19-3518-2316
Skype: Marin.van.Heel
email: marin.vanheel(A_T)gma
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