One more remark on the camera correction issue... The camera correction does actually matter quite a bit, especially where it concerns the movie alignments in the early phases of processing, as Tanaka mentioned too. What I want to point out here is that the effect of the camera correction needs to be judged directly at the camera level by the FRC, or better still, by the FRI (https://arxiv.org/abs/2009.03223) and not at the end of a long processing chain where the influence of tons of unrelated intermediate processing decisions have been made. (Yes the quality of the shoes of a football player has an influence on who wins the game, but blaming the outcome of a game on the quality of shoes of the football player..., well... ). Since the camera correction significantly influences the movie alignment procedures, and assuming all processing is done adhering to the appropriate sampling and processing rules, the main influence on the 3D output of the game will be in the number of particles effectively available for 3D reconstruction. That number will affect the FSC (Harauz & van Heel 1986), yes, but less than it will affect the R-weighted Fourier Shell Information FSI (https://arxiv.org/abs/2009.03223)! Bottom line: take care of your metrics and stick to the rules and you will win the game!
Two more pennies on the issue, Marin On Mon, Sep 14, 2020 at 3:50 PM Thomas Cleveland <thomas.clevel...@gmail.com> wrote: > Thanks everyone for the tool suggestions and comments. I particularly > enjoyed the correction of Mars rover photos as an example. > > Summary: > > 1. sum_all_tiffs in cisTEM > 2. relion_estimate_gain > 3. camera-correction command in IMAGIC ( > https://www.nature.com/articles/srep10317) > > Tom > > On Mon, Sep 14, 2020 at 2:11 PM Thomas Cleveland < > thomas.clevel...@gmail.com> wrote: > >> Hi all, >> >> I've heard of folks averaging an entire set of unaligned movie files in >> order to make a new gain reference after data collection. Can anyone >> comment on how well this works? Is there any command that does it in a >> really simple way? (I realize it would not be too hard to just loop through >> and average all the movies, but thought I would ask, in case this is >> already implemented in a tool somewhere). >> >> Best, >> Tom >> > > ------------------------------ > > To unsubscribe from the CCPEM list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCPEM&A=1 > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
