Hi,
I have uninstalled ccp4 from my laptop and reinstalled a recent version. I
am unable to access my old run and also my project directory looks empty. I
am able to individually connect to each project. Since I have multiple
projects is there a way to connect all my project at once
Thanking y
Hi all,
I have determined the structure of a protein at 3Å resolution. The protein
has 6 chains in the asymmetric unit. In all these 6 chains I see a density
near tyrosine which is at a distance of 2.1 from the OH group of tyrosine.
Since this distance is short for a hydrogen bond we are skeptical
t;
>
> Il giorno mar 30 giu 2020 alle ore 12:52 Lumbini Yadav <
> lumbin...@gmail.com> ha scritto:
>
>> Hi Daniele,
>> Do you see Fo-Fc density near disuphide bond where you are trying to fit
>> acetone?
>>
>> On Tue, Jun 30, 2020 at 3:28 PM Daniele Ve
Hi Daniele,
Do you see Fo-Fc density near disuphide bond where you are trying to fit
acetone?
On Tue, Jun 30, 2020 at 3:28 PM Daniele Veggi
wrote:
> Dear CCP4bb,
>
> I'm trying to insert an Acetone molecule between two cysteine residues in
> coot or modifying the pdb. I'm working on this modifi
Dear Rajnesh,
Why don't you try refining the 4 domain structure initially. Once the R
factor and R free value is sufficiently low then try to see if there is any
density build up for domain 5.
This has atleast worked for me.
On Wed, 12 Feb 2020, 12:53 Rajnesh Kumari Yadav, wrote:
> Hello everyon
Dear Tarique,
Using Blend will be a good idea as it gives multiple merging statistics for
different data combination. This helps one in deciding which set of data to
use to proceed ahead for MR
Regards
lumbini
On Wed, Jan 15, 2020 at 7:38 PM Firdous Tarique
wrote:
> Hi.
>
> I have collected mul
Dear Amala,
1. Hampton provides a precrystallisation screening kit which can be used to
determine the protein concentration to be used for crystallisation.
2. Also if you observe the screening crystallisation plate, according to
what I follow, at least there should be minimum 60 % of conditions w
Sudipta to link this amino acid you have to renumber the residues before
and after the modified position where residue is added such that it
accepts the residues as part of chain.
Also you should give similar chain id to modified residue, as the chain in
which you want to merge
Hope this helps.
t a metal or a sulphate or...
>
> But that still doesnt explain WHAT it is . Sorry not to be more help..
>
> Eleanor
>
> On Wed, 10 Jul 2019 at 11:32, Lumbini Yadav wrote:
>
>> No I am using ccp4i. I tried doing SAD refinement in refmac and the
>> output image is a
; Eleanor
>
> On Wed, 10 Jul 2019 at 09:32, Lumbini Yadav wrote:
>
>> I have soaked my crystals in sodium dithionite a reducing agent. I have
>> not done mass spec but sequence is confirmed
>>
>> On Tue, Jul 9, 2019 at 9:26 PM Bonsor, Daniel
>> wrote
A Bonsor PhD.
>
> Sundberg Lab
>
> Institute of Human Virology
>
> University of Maryland, Baltimore
>
> 725 W Lombard Street N370
>
> Baltimore
>
> Maryland
>
> MD 21201
>
> Tel: (410) 706-7457
>
>
>
>
>
> *From:* CCP4 bulletin board
---
> *From: *176a9d5ebad7-dmarc-requ...@jiscmail.ac.uk
> *To: *CCP4BB@JISCMAIL.AC.UK
> *Sent: *Tuesday, July 9, 2019 4:48:32 PM
> *Subject: *Re: [ccp4bb] Fo-Fc density close to cysteine residue
>
> Any anomalous diffraction?
>
> On Tue, 9 Jul 2019 at 10:32, Lumbini Yad
Dear all,
We have found a huge Fo-Fc density close to cysteine residue (see attached
image) in the structure with resolution of 1.2A. In the crystallization
condition, we have PEG 3350, Potassium phosphate monobasic, glycerol and
protein was in Tris and NaCl. Before freezing the crystals were so
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