Dear Rajnesh, Why don't you try refining the 4 domain structure initially. Once the R factor and R free value is sufficiently low then try to see if there is any density build up for domain 5. This has atleast worked for me.
On Wed, 12 Feb 2020, 12:53 Rajnesh Kumari Yadav, <rajn...@rcb.res.in> wrote: > Hello everyone, > > I am working on a protein which have 5 domain in it, we have its 4 domain > structure and 3.0 Angstrom data of 5 domain protein crystal. While doing > the Molecular replacement we got solution with four domain with good > electron density and space for last domain without any density. When we > tried Molecular replacement with the missing domain (modelled) only we got > solution shows density for this missing domain but not able see electron > density for rest four domain even though room for 4 domain was visible in > the solution. I need help or suggestion for this issue. > > ######################################################################## > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
