Hi Daniele, Do you see Fo-Fc density near disuphide bond where you are trying to fit acetone?
On Tue, Jun 30, 2020 at 3:28 PM Daniele Veggi <veggidani...@gmail.com> wrote: > Dear CCP4bb, > > I'm trying to insert an Acetone molecule between two cysteine residues in > coot or modifying the pdb. I'm working on this modified molecule where the > disulfide bridge was chemichally opened and inserted an acetone molecule > between the two cysteine. > > MS data confirm this modification (98% of molecules modified) but I can > not insert the acetone covalently linked to the S of both cysteine > (S-ACN-S). > Maybe what I think and what I am doing is not the right solution (the > disulfide bridge is still alive?) anyway i would like to know how to link > the acetone to the two cysteine > To better understand what I mean in attach you find a screenshot > > I thank in advance all those who will have the courtesy to make a > suggestion > > Daniele Veggi > > ------------------------------ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
