Hi Gloria,
I have extensively used SUMO and echoing Artem's sentiment. SUMO has worked
well in my hands for most instances. The additional benefit is that,
assuming you use His-SUMO-target, following His-tag affinity purification,
you can cleave the SUMO tag, followed by reverse IMAC (i.e., collec
Hi Gloria!
What’s the point of publishing a nice paper if nobody reads it? *Sigh* (see
reference below, we’re happy to share vectors).
So yes, SUMO fusions have definitely worked for us; this strategy is our first
choice for expressing any new soluble protein, and we use it for a lot of
membra
Affirmative on all these questions:
SUMO works well
SUMO protease is super easy to make in house
As to which one - regular SUMO for E. coli and SUMO* for mammalian or
insect cell systems because regular SUMO may get cleaved by the intrinsic
protease. SUMO* is a triple mutant, with corresponding m
Hi All,
There is a bit of information in the crystallization conditions that I
would like to comment on.
Crystals grow in a variety of conditions, including one that contains
Na-Cacodylate buffer (#3 in the list).
When this is the case, the cacodylate-containing conditions should be
dropped from fu
Hello friends, Yet another kinda off topic question from me. I am looking
into SUMO tags for my protein of interest to help solubility. Has this
worked for you? If so which SUMO and protease do you use? Can the
protease be made in house? So far I am looking at the pET SUMO system. Is
this th
Dear all,
We have an open temporary position for a beamline scientist at MicroMAX at MAX
IV. MicroMAX is a versatile MX beamline focusing on serial and time-resolved
crystallography that hosted its first experiments in this area during spring
2024. The beamline is an exciting stage with new fun
Dear colleagues,
The joint DFG Research Training Group 2887 “VISION - Visualization and Imaging
in Viral Infection” between Universität zu Lübeck and Universität Hamburg
invites applications for
Eleven (11) Ph.D. Positions
to start at October 1st, 2025, with a salary of 65% grade 13 (TV-L E13)
The team “Structural Biology of Molecular Machines” at the IGBMC in Strasbourg,
France, is looking for an enthusiastic and highly motivated PhD student. One of
the main research axis in the group is the dynein motor protein, a complex and
fascinating molecular machine involved in essential cellu
The team “Structural Biology of Molecular Machines” at the IGBMC in
Strasbourg, France, is looking for an enthusiastic and highly motivated PhD
student. One of the main research axis in the group is the dynein motor
protein, a complex and fascinating molecular machine involved in essential
cellular
