Hi Gloria!

What’s the point of publishing a nice paper if nobody reads it? *Sigh* (see 
reference below, we’re happy to share vectors).

So yes, SUMO fusions have definitely worked for us; this strategy is our first 
choice for expressing any new soluble protein, and we use it for a lot of 
membrane proteins as well.  We make a doubly tagged version of the yeast UD1 
protease, and it works great (SO much more efficient than TEV). 

Pat
 
Weeks, S. D.; Drinker, M.; Loll, P. J. Ligation Independent Cloning Vectors for 
Expression of SUMO Fusions. Protein Expression and Purification 2007, 53 (1), 
40–50. https://doi.org/10.1016/j.pep.2006.12.006.


> On Dec 12, 2024, at 11:36 AM, Gloria Borgstahl <gborgst...@gmail.com> wrote:
> 
> Hello friends, Yet another kinda off topic question from me.  I am looking 
> into SUMO tags for my protein of interest to help solubility.   Has this 
> worked for you?  If so which SUMO and protease do you use?  Can the protease 
> be made in house?  So far I am looking at the pET SUMO system.  Is this the 
> best?  Thank you in advance. Gloria 
> 
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---------------------------------------------------------------------------------------
Patrick J. Loll, Ph. D.  (he, him, his)
Professor of Biochemistry & Molecular Biology
Drexel University College of Medicine
Room 10-102 New College Building
245 N. 15th St., Mailstop 497
Philadelphia, PA  19102  USA

(215) 762-7706
pjl...@gmail.com
pj...@drexel.edu

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