Hi Gloria! What’s the point of publishing a nice paper if nobody reads it? *Sigh* (see reference below, we’re happy to share vectors).
So yes, SUMO fusions have definitely worked for us; this strategy is our first choice for expressing any new soluble protein, and we use it for a lot of membrane proteins as well. We make a doubly tagged version of the yeast UD1 protease, and it works great (SO much more efficient than TEV). Pat Weeks, S. D.; Drinker, M.; Loll, P. J. Ligation Independent Cloning Vectors for Expression of SUMO Fusions. Protein Expression and Purification 2007, 53 (1), 40–50. https://doi.org/10.1016/j.pep.2006.12.006. > On Dec 12, 2024, at 11:36 AM, Gloria Borgstahl <gborgst...@gmail.com> wrote: > > Hello friends, Yet another kinda off topic question from me. I am looking > into SUMO tags for my protein of interest to help solubility. Has this > worked for you? If so which SUMO and protease do you use? Can the protease > be made in house? So far I am looking at the pET SUMO system. Is this the > best? Thank you in advance. Gloria > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 --------------------------------------------------------------------------------------- Patrick J. Loll, Ph. D. (he, him, his) Professor of Biochemistry & Molecular Biology Drexel University College of Medicine Room 10-102 New College Building 245 N. 15th St., Mailstop 497 Philadelphia, PA 19102 USA (215) 762-7706 pjl...@gmail.com pj...@drexel.edu ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
