A POSITION FOR A POST DOCTORAL FELLOW in Protein Crystallography/Structural
Biology/Cell Biology in University of Oulu, Finland
We open a position for a post-doctoral fellow in the Faculty of Biochemistry
and Molecular Medicine
(University of Oulu, Finland) on a project that focuses on Golgi
Gl
Interesting! So chelation alone wouldn't help because the (non GMO) apples
have PPO to catalyze the oxidation in the absence of metals.
But the ascorbate keeps things reduced, and the acid raises
the midpoint potential of phenolics (a proton is given off
going from -OH to =O) making them weaker re
On 07/03/14 17:05, Edward A. Berry wrote:
I see, L-dopa is a phenolic (o-quinolic actually) compound, same as
what gets oxidized
in sliced apples to turn them brown.
At least, that's the way it used to be.
http://www.okspecialtyfruits.com/arctic-apples/about-our-nonbrowning-apples
"Truly nonb
I see, L-dopa is a phenolic (o-quinolic actually) compound, same as what gets
oxidized
in sliced apples to turn them brown. Juice squeezed from half a lemon works
great:
citrate to chelate metals which catalyze the oxidation, ascorbate to reduce
everything back to colorless, and the low pH gives
Hi Everyone,
Can someone please help me figure out what's wrong with my SelMet
incorporation protocol. My protein of interest is 500 amino acids with 20
of these being Methionines. However mass spec data show that only two of
these 20 Methionine sites are partially labelled with SelMet. The follow
Dear Jacob,
Please check the following references:
http://www.ncbi.nlm.nih.gov/pubmed/8771063
http://www.ncbi.nlm.nih.gov/pubmed/9251095
>From some limited reading of another paper, it seems like the dark solution
>could be the conversion of L-Dopa into melanin, which is accelerated in
>alkal
No direct experience, but I think that there is some literature
supporting increased stability of L-DOPA drug formulations with
ascorbate.
- John
On Thu, Jul 3, 2014 at 1:34 PM, Keller, Jacob wrote:
> Dear Crystallographers,
>
> Does anyone have experience with co-crystallization of a protein wi
levodopa solutions are stable in refrigerator conditions for a day.
You make as much you want and prepare fresh everyday.
there is no easy ways
On Thu, Jul 3, 2014 at 10:34 AM, Keller, Jacob
wrote:
> Dear Crystallographers,
>
> Does anyone have experience with co-crystallization of a protein wi
Dear Crystallographers,
Does anyone have experience with co-crystallization of a protein with L-Dopa?
In my hands, the L-Dopa degrades in water to a dark solution within 10 minutes.
I am thinking of trying some reductants and metal chelation, but a verified
successful formula would be much appr
Hi everyone
Thanks for your suggestions.
I found a cooler in the lab that is exactly to the size of the 24 well
regular crystallography plates.
It is for the crystallographer before my advisor got his position. Probably
they were using it for the same purpose at the time that cryo cooling
technique
Hi Meisam,
Boxes sold here keep temperature very stable (+/- 1 deg) for several days:
http://greenboxsystems.com/. I've used them successfully to transport LCP
samples, keeping them between 18 and 20 deg C, monitoring temperature with an
EL-USB temperature logger
(http://www.lascarelectronics.c
On Thu, Jul 3, 2014 at 6:53 AM, Dirk Kostrewa
wrote:
> yes - unfortunately, in my hands, phenix.xtriage reads the XDS_ASCII.HKL
> intensities as amplitudes, producing very different output statistics,
> compared both to the XDS statistics and to an mtz file with amplitudes
> created from that XDS
Hi Tim,
yes - unfortunately, in my hands, phenix.xtriage reads the XDS_ASCII.HKL
intensities as amplitudes, producing very different output statistics,
compared both to the XDS statistics and to an mtz file with amplitudes
created from that XDS file. I've contacted a phenix developer a few
we
Hi Dirk,
that would truely be very sad - the XDS file format is such a beautiful,
self-contained and well documented format for diffraction data that a
misinterpretation should really not happen.
Cheers,
Tim
On 07/03/2014 01:42 PM, Dirk Kostrewa wrote:
> ... and please check, whether phenix.xtri
... and please check, whether phenix.xtriage recognized the input data
as intensities or as amplitudes.
In case of doubt, convert the intensives first into an mtz file with Fs
instead of Is and run phenix.xtriage on the mtz file.
Best regards,
Dirk.
Am 03.07.2014 13:36, schrieb Tim Gruene:
H
Hi Yamei,
did you by any chance feed the output file from XDS into xtriage? It
would indicate the data were twinned even for a near perfect insulin
test crystal. After discussion with the developers I understand that
phenix does not seem to handle unmerged data well.
With phenix.xtriage V. phenix
Hi Wenhe,
The ccp4 program "sortwater" will perform this task.
Philip
On Wed, Jul 2, 2014 at 11:42 PM, Wenhe wrote:
> Dear CCP4BB members,
>
> I want to keep the chain ID of water molecules the same as their
> interacting protein molecule. For example, I have two protein molecules in
> the st
Hi Yamei,
A possible explanation is that the actual space group is P4(2) but the data
are perfectly hemihedrally twinned, which makes the crystal appear to
possess 422 point group symmetry. No twin operators are found because
merohedral twinning is not possible in crystals with true 422 symmetry.
Dear Wehne,
Have you looked at the CCP4 program "watertidy" … I think it does what you want
(but I have not used it myself).
Andrew
On 3 Jul 2014, at 04:42, Wenhe wrote:
> Dear CCP4BB members,
>
> I want to keep the chain ID of water molecules the same as their interacting
> protein molecu
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