Re: [ccp4bb] "Quick soak" method

I also solved a structure on our home CuKa source by SIRAS with 20-60sec in 0.5-1M KI. JPK ==Original message text=== On Tue, 25 Sep 2007 6:55:28 pm CDT James Whisstock wrote: Hi - sorry - rather than iodine I meant to say we had had success with Potassium Iodide (1M f

Re: [ccp4bb] "Quick soak" method

Hi - sorry - rather than iodine I meant to say we had had success with Potassium Iodide (1M for 20 seconds)! Cheers James [EMAIL PROTECTED] wrote:> > Hi, > > I do not use their method as such - however, I love heavy atom soaks and > do them any time I can, so I've got very similar experience

Re: [ccp4bb] "Quick soak" method

Hi, I do not use their method as such - however, I love heavy atom soaks and do them any time I can, so I've got very similar experiences in the past. Heavy atoms can bind very quickly even from quite dilute solutions - the quickest I've ever soaked (and got useful data) was sodium chloroplatinat

Re: [ccp4bb] "Quick soak" method

Hi Derek We have had success with iodine - 20 seconds soak. J Derek Logan <[EMAIL PROTECTED]> wrote:> > Hi Uwe, > > Just what I wanted to hear, and with a limited set of compounds too! > Follow-up question: what are these 4-6 most successful compounds? > Thanks also for the tips on monitoring

Re: [ccp4bb] Alignment question

Thanks a lot for Francesco, Ethan, and Jessica. Yes, it is ESPrit. For unknown reasons, when I use keywords: "sequence + alignment + tools" in google that server never showed up. It is also worthy to look at the other servers suggested in the reply given below: Francesco Charlie Bond has

Re: [ccp4bb] Alignment question

On Tuesday 25 September 2007 14:22, Ibrahim M. Moustafa wrote: > > I'm looking for a webserver that can take the alignment and > produce a nice figure with the alignment annotated with sec. structure on top, I use TeXShade

[ccp4bb] Alignment question

Hi all, I'm looking for a webserver that can take the alignment and produce a nice figure with the alignment annotated with sec. structure on top, and draw boxes around conserved residues. Long time ago I had used a server doing exactly what I described; unfortunately, I lost the informa

[ccp4bb] Announcement:MIFit+ released

We are pleased to announce the release of a significantly updated version of the MIFit+ software. MIFit+ contains the MIFit crystallographic model-fitting and display program as well as the MIExpert application for managing crystallographic structure solution software. MIFit contains a 'ligand dict

[ccp4bb] Pymol installation

Dear all. We have had success installing Pymol on any 32 bits PC, but we are having some troubles with 64 bits. The OS is linux ubuntu kernel: 2.6.15-29-amd64-generic x86_64 The pymol version is: pymol-0_99rc6-bin-linux-x86-glibc23.tgz The error message is: freeglut (./pymol.exe): ERROR: I

Re: [ccp4bb] "Quick soak" method

There's a nice databank out there in the world wild web: http://www.sbg.bio.ic.ac.uk/had/ Juergen Derek Logan wrote: Hi Uwe, Just what I wanted to hear, and with a limited set of compounds too! Follow-up question: what are these 4-6 most successful compounds? Thanks also for the tips on

Re: [ccp4bb] "Quick soak" method

Hi, I've had a couple of successful cases: 1) Uranyl acetate soak to get starting phases at 6.0A combined with a gold derivative at 3.8A. Both quick soaks of about 10-20 mins in 5-10 mM on crystals less than 100 microns. 2) Mercuric chloride soak of 10 mM for 10 mins to get starting phases

Re: [ccp4bb] "Quick soak" method

Hi Uwe, Just what I wanted to hear, and with a limited set of compounds too! Follow-up question: what are these 4-6 most successful compounds? Thanks also for the tips on monitoring thw soaking Derek On Sep 25, 2007, at 13:23, Uwe Mueller wrote: Dear Derek, we are using the quick-soak me

Re: [ccp4bb] "Quick soak" method

You may also wish to take a look in Dauter and Nagem's quick cryo soaking for introducing anomalous scattering. 1. Acta Crystallogr D Biol Crystallogr. 2001 Jul;57(Pt 7):996-1002. 2. Methods Enzymol. 2003;374:120-37. 3. Acta Crystallogr D Biol Crystallogr. 2005 Aug;61(Pt 8):1022-30 HTH [ ]s -

[ccp4bb] Fall microbeam time still available at MacCHESS

Protein crystals smaller than 30 micrometers in diameter can be difficult to handle and produce weak diffraction. We have one 48 hour slot left in our Fall running schedule for microcrystallography. An 18 micrometer beam is an excellent way to improve signal by delivering photons only whe

[ccp4bb] SRS AP50 (April - December 2008) Open for Applications

Dear SRS Users, Applications are now invited for beam-time in allocation period 50 (April - December 2008) on PX station 10.1 at the SRS. The deadline for submission of proposals is Thursday 1st November. Further details on how to apply for beamtime can be found here :- http://www.srs.ac.uk/sr

[ccp4bb] Crystallographic Post-doctoral Position in Champaign-Urbana, IL

An NIH funded post-doctoral position is available to work as part of a multi-disciplinary effort on structure determination of proteins involved in antibiotic biosynthesis (Science 311, 1464-1467). Candidate should have a background in standard protein purification, synchrotron data collectio

Re: [ccp4bb] CNS setup file for bash users?

Many thanks to Kay, Navraj and to Stuart! Apparently, the CNS setup script for bash comes with the CNS installation but is installed as an "invisible" file, which is rather strange ... Best regards, Dirk. Am 24.09.2007 um 10:34 schrieb Dirk Kostrewa: Dear colleagues, although not a CCP

Re: [ccp4bb] "Quick soak" method

Dear Derek, we are using the quick-soak method frequently at the BESSY-MX beamlines. So far we were able to obtain phase information in terms of MAD/SAD- phases in all cases which we tried. The starting set-up is the screen over the 4-6 most successful heavy atom compounds at 5-10mM concentrat

[ccp4bb]

Re: [ccp4bb] mosflm orientation matrix and symmetry

Hi Bryan, So, there are two answers to this question. If you are changing the spacegroup within the same lattice type (e.g. tP) then it is fine (this is probably what you mean by crystal system) - though you can do this by just running reindex "symm whatever" afterwards. If you want to change from

[ccp4bb] hanging drop tape for crystallisation

Sorry for the non-ccp4 post but I thought people on this bulletin board may be able to help. We have been setting up crystallisation screens using a Mosquito robot with 96 well-format 3M sealing tape with hanging drop circles embedded in the tape. This works really well but we've just discove

[ccp4bb] source for hanging drop 3M tape

Sorry for the non-ccp4 post but I thought people on this bulletin board may be able to help. We have been setting up crystallisation screens using a Mosquito robot with 96 well-format 3M sealing tape with hanging drop circles embedded in the tape. This works really well but we've just dis

[ccp4bb] "Quick soak" method

Hi, I'd like to find out how successful the "quick soak" method for heavy atom derivatisation proposed by Radaev and Sun: Sun PD, Radaev S, Kattah M. Generating isomorphous heavy-atom derivatives by a quick-soak method. Part I: test cases. Acta Cryst. 2002. D58:1092-1098. has been in co