Thank you everybody for their nice suggestions.. On Thu, May 23, 2013 at 7:39 PM, Matthew BOWLER <mbow...@embl.fr> wrote:
> I keep sending mails by accident today - apologies for the spam. The last > sentence of my should read: > > This could of course be due to too high a concentration of mother liquor > but quite often it occurs at relative humidity values where the > concentration of the mother liquor components will not have increased by > very much. Cheers, Matt. > > > On 2013-05-23 15:32, Ed Pozharski wrote: > >> Matt, >> >> with this technique, how do you prevent crystal from drying up (other >> than "doing it fast")? I know Thorne's group does this trick under oil. >> If you take no extra precautions, do you have an estimate of how often >> diffraction is destroyed by this? >> >> On the other hand, it's quite possible that what destroys resolution >> when crystals dry up is increase in concentration of non-volatile mother >> liquor components, which shouldn't be happening here to the same degree. >> >> Cheers, >> >> Ed. >> >> On Thu, 2013-05-23 at 14:38 +0200, Matthew BOWLER wrote: >> >>> Hi Faisal, >>> if your solvent channels are smaller than 40A in the largest dimension >>> (most are) you can use a mesh loop to pick up the crystal and then wick >>> away all of the mother liquor. You can then flash cool your crystal >>> without having to transfer the crystal to another solution. Good luck, >>> Matt >>> >> > -- > Matthew Bowler > Synchrotron Science Group > European Molecular Biology Laboratory > BP 181, 6 rue Jules Horowitz > 38042 Grenoble Cedex 9 > France > ==============================**===================== > Tel: +33 (0) 4.76.20.76.37 > Fax: +33 (0) 4.76.88.29.04 > > http://www.embl.fr/ > ==============================**===================== > -- Regards Faisal School of Life Sciences JNU
