L-proline works well with ammonium sulfate: http://www.ncbi.nlm.nih.gov/pubmed/22868767
Sent from Jack's iPad On May 23, 2013, at 4:42 AM, "Faisal Tarique" <faisaltari...@gmail.com> wrote: > > Dear all > > Can anybody tell me the appropriate cryo condition for the crystals obtained > in 2M Ammonium sulphate and tris pH8.5..I tried to add 10% glycerol to it but > still the ice ring is forming.. > > Thanx in advance > -- > Regards > > Faisal > School of Life Sciences > JNU