L-proline works well with ammonium sulfate:

http://www.ncbi.nlm.nih.gov/pubmed/22868767


Sent from Jack's iPad

On May 23, 2013, at 4:42 AM, "Faisal Tarique" <faisaltari...@gmail.com> wrote:

> 
> Dear all
> 
> Can anybody tell me the appropriate cryo condition for the crystals obtained 
> in 2M Ammonium sulphate and tris pH8.5..I tried to add 10% glycerol to it but 
> still the ice ring is forming..
> 
> Thanx in advance
> -- 
> Regards
> 
> Faisal
> School of Life Sciences
> JNU

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