Hi Faisal,
if your solvent channels are smaller than 40A in the largest dimension
(most are) you can use a mesh loop to pick up the crystal and then wick
away all of the mother liquor. You can then flash cool your crystal
without having to transfer the crystal to another solution. Good luck,
Matt
On 2013-05-23 11:42, Faisal Tarique wrote:
Dear all
Can anybody tell me the appropriate cryo condition for the crystals
obtained in 2M Ammonium sulphate and tris pH8.5..I tried to add 10%
glycerol to it but still the ice ring is forming..
Thanx in advance--
Regards
Faisal
School of Life Sciences
JNU
--
Matthew Bowler
Synchrotron Science Group
European Molecular Biology Laboratory
BP 181, 6 rue Jules Horowitz
38042 Grenoble Cedex 9
France
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