30% glycerol or 25% glucose should be sufficient for 1-2 M ammonium sulfate.
Roger Rowlett On May 23, 2013 5:42 AM, "Faisal Tarique" <faisaltari...@gmail.com> wrote: > > Dear all > > Can anybody tell me the appropriate cryo condition for the crystals > obtained in 2M Ammonium sulphate and tris pH8.5..I tried to add 10% > glycerol to it but still the ice ring is forming.. > > Thanx in advance > -- > Regards > > Faisal > School of Life Sciences > JNU >