Dear All, Thank you so much. Because the data quality is not so good (P1 space group, Rmerge 0.19, redundancy 3.9). I would like to try all the methods one by one to see which is better for my case. Thanks again.
On Wed, Jul 13, 2011 at 8:01 AM, Soisson, Stephen M < stephen_sois...@merck.com> wrote: > ** > Seconding David's suggestion, I have had this issue on several occasions > and I would highly recommend using SHARP. In my experience, SHARP does a > superior job handling this type of data. > > Best of luck- > > Steve > > > ------------------------------ > *From:* CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] *On Behalf Of > *David Veesler > *Sent:* Tuesday, July 12, 2011 9:21 PM > *To:* CCP4BB@JISCMAIL.AC.UK > *Subject:* Re: [ccp4bb] how to combine the experimental phase and > molecular replacement phase > > Hi Jiamu, > to complete the Juergen answer, that's true that the MR-SAD approach is > very powerful and can definitely help in your case. You can use Sharp and > add the information from your partial molecular replacement solution encoded > as HL coefficients and do a MR-SAD phasing. > Here is the procedure > http://www.globalphasing.com/sharp/manual/chapter4.html#ExternalPhaseInformation > Cheers > David > > > Le 12 juil. 2011 à 18:08, Jiamu Du a écrit : > > Dear All, > I am now working on a low resolution phase determination (around 3.3 A with > Se anomalous signal around 3.8 A). > I can find the Se site and get the phase, but the density map is not so > good. > Some part of the protein (about 1/3) has a homologue model which is also > can be found using Phaser. The homologue region has a good map while other > region only show a poor map. > I think the combination of experimental phase and MR phase might improve > the map. Is there anybody can help find which program can work on this? > > Thanks. > -- > Jiamu Du, Ph.D. > Postdoctoral Research Fellow > Laboratory of Structural Biology > Memorial Sloan-Kettering Cancer Center > RRL 269, 430 E 67th Street > New York, NY, 10021 > E-mail: d...@mskcc.org > Tel: (217) - 417 - 9897 > > > -- > This message has been scanned for viruses and > dangerous content by *MailScanner* <http://www.mailscanner.info/>, and is > believed to be clean. > > > > -- > This message has been scanned for viruses and > dangerous content by *MailScanner* <http://www.mailscanner.info/>, and is > believed to be clean. > > Notice: This e-mail message, together with any attachments, contains > information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, > New Jersey, USA 08889), and/or its affiliates Direct contact information > for affiliates is available at http://www.merck.com/contact/contacts.html) > that may be confidential, > proprietary copyrighted and/or legally privileged. It is intended solely > for the use of the individual or entity named on this message. If you are > not the intended recipient, and have received this message in error, > please notify us immediately by reply e-mail and then delete it from > your system. > > -- Jiamu Du, Ph.D. Postdoctoral Research Fellow Laboratory of Structural Biology Memorial Sloan-Kettering Cancer Center RRL 269, 430 E 67th Street New York, NY, 10021 E-mail: d...@mskcc.org Tel: (217) - 417 - 9897
