Hi Jiamu, OASIS may help solving your problem. We have example on solving an originally unknown protein (1086 residue in the AU) at 3.3A resolution with Se-SAD data (redundancy=3.3). The process involved SAD-iteration and MR-iteration with OASIS. If you need help, we can discuss off list.
Cheers Hai-fu > On Tue, Jul 12, 2011 at 6:08 PM, Jiamu Du <jiam...@gmail.com> wrote: > >> Dear All, >> I am now working on a low resolution phase determination (around 3.3 A >> with Se anomalous signal around 3.8 A). >> I can find the Se site and get the phase, but the density map is not so >> good. >> Some part of the protein (about 1/3) has a homologue model which is also >> can be found using Phaser. The homologue region has a good map while other >> region only show a poor map. >> I think the combination of experimental phase and MR phase might improve >> the map. Is there anybody can help find which program can work on this? >> >> Thanks. >> -- >> Jiamu Du, Ph.D. >> Postdoctoral Research Fellow >> Laboratory of Structural Biology >> Memorial Sloan-Kettering Cancer Center >> RRL 269, 430 E 67th Street >> New York, NY, 10021 >> E-mail: d...@mskcc.org >> Tel: (217) - 417 - 9897 >> >> >
