Dear Jiamu,
Try out MRSAD protocol of Auto-Rickshaw
(http://www.embl-hamburg.de/Auto-Rickshaw). You can start this just by
providing your selenomet
data, starting model, sequence information and space group on the
Auto-Rickshaw server. It would do substructure determination based on
your model phase, experimental phasing, phase combination, density
modification, model building and refinement of the built model.
Iteratively it improves phases and complete your model as much as
possible.
Best
Santosh
Quoting "Jiamu Du" <jiam...@gmail.com>:
Dear All,
I am now working on a low resolution phase determination (around 3.3 A with
Se anomalous signal around 3.8 A).
I can find the Se site and get the phase, but the density map is not so
good.
Some part of the protein (about 1/3) has a homologue model which is also can
be found using Phaser. The homologue region has a good map while other
region only show a poor map.
I think the combination of experimental phase and MR phase might improve the
map. Is there anybody can help find which program can work on this?
Thanks.
--
Jiamu Du, Ph.D.
Postdoctoral Research Fellow
Laboratory of Structural Biology
Memorial Sloan-Kettering Cancer Center
RRL 269, 430 E 67th Street
New York, NY, 10021
E-mail: d...@mskcc.org
Tel: (217) - 417 - 9897
Santosh Panjikar, Ph.D.
Staff Scientist
EMBL-Hamburg Outstation
C/o DESY
Notkestrasse 85
22603 Hamburg (Germany)
panjikar at embl-hamburg.de
http://www.embl-hamburg.de/~panjikar
