Very good. Now how many atoms are present in your input PDB file?
We'll be able to help you shortly. Fred. Vandana Kukshal wrote:
i am using restrain refmac for refine ment and coot for fiting its resolution range is 116.248 -3.00 number of used reflections 20215 the R factor is 27.7 and R free is 27.0 and if i am doing further restrain refinement with 0.03 geometry weight R factor is going down to 25.5 but R free is increasing to 30.5 should i go for further refinemnet
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