Rob, A couple of years ago my colleague and I "mined" the crystallization data in REMARK 280 from 3939 entries in the PDB. In 109 entries, glycerol came out as the ingredient with the highest concentration that was measured with a % (percent) sign i.e. including '%', '%W/V', '%V/V' etc.
The average concentration of these 109 entries was 15.5% Many of these appeared to be "salting in" conditions. So 10% glycerol should be no problem - it may be close to the overall average (which we don't know). Patrick On 8/25/07, Rob Gruninger <[EMAIL PROTECTED]> wrote: > > > > Dear All, > I am working with a protein that requires 10% glycerol throughout the > purification to keep it soluble. I have been very worried that having > glycerol in my protein solution when I am trying to crystallize it will > prevent me from obtaining crystals. I am curious to see if others have > successfully obtained crystals of proteins that required glycerol to keep > them soluble and if so what concentration of glycerol could you use? > Should I be concerned about this or am I worrying too much? > Thanks for your input > Rob > > -- > Robert J.Gruninger B.Sc. > PhD Student > Department of Chemistry & Biochemistry > University of Lethbridge, 4401 University Dr. > Lethbridge, AB, Canada, T1K 3M4 > Phone:(403)329-2746 > Fax:(403)329-2057 >
