We've grown crystals of the cytochrome bc1 complex in the presence of glycerol.
I think as high as 25% in the initial droplet (protein in 50% glycerol mixed with equal volume of precipitant), but that was diluted somewhat by reverse vapor diffusion. Glycerol tends to increase the solubility in our case, so the PEG concentration we had to use in those experiments was higher than in later glycerol-free setups. Ed Rob Gruninger wrote:
Dear All, I am working with a protein that requires 10% glycerol throughout the purification to keep it soluble. I have been very worried that having glycerol in my protein solution when I am trying to crystallize it will prevent me from obtaining crystals. I am curious to see if others have successfully obtained crystals of proteins that required glycerol to keep them soluble and if so what concentration of glycerol could you use? Should I be concerned about this or am I worrying too much? Thanks for your input Rob
