I have crystallized a refolded membrane protein in presence of Glycerol. It did not seem to affect crystallizability and speed of crystallization. If you have no luck in presence of glycerol, try to lower the glycerol concentration without compromising on the stability of the protein (buffer exchange). Remember that Glycerol concentration is going to drop when you mix it with reservoir solution, depending upon the volume ratio that you use. Glycerol is known to delay crystallization a bit. There are cases where the quality of crystals are improved. subjective! Good luck Kumar
>===== Original Message From Rob Gruninger <[EMAIL PROTECTED]> ===== >Dear All, >I am working with a protein that requires 10% glycerol throughout the >purification to keep it soluble. I have been very worried that having >glycerol in my protein solution when I am trying to crystallize it will >prevent me from obtaining crystals. I am curious to see if others have >successfully obtained crystals of proteins that required glycerol to keep >them soluble and if so what concentration of glycerol could you use? >Should I be concerned about this or am I worrying too much? >Thanks for your input >Rob > >-- >Robert J.Gruninger B.Sc. >PhD Student >Department of Chemistry & Biochemistry >University of Lethbridge, 4401 University Dr. >Lethbridge, AB, Canada, T1K 3M4 >Phone:(403)329-2746 >Fax:(403)329-2057 Dept. of Biochemistry, Cellular and Molecular Biology, Walters Life Science, # 406, University of Tennessee, TN, Knoxville, USA
