We have crystallised many things with 10% Glycerol. If % is high enough, there is also often the added bonus in that the crystals are naturally cryoprotected!
J Edward Berry <[EMAIL PROTECTED]> wrote:> > We've grown crystals of the cytochrome bc1 complex in the > presence of glycerol. > > I think as high as 25% in the initial droplet (protein in 50% > glycerol mixed with equal volume of precipitant), > but that was diluted somewhat by reverse vapor diffusion. > Glycerol tends to increase the solubility in our case, > so the PEG concentration we had to use in those experiments > was higher than in later glycerol-free setups. > Ed > > Rob Gruninger wrote: > >> Dear All, >> I am working with a protein that requires 10% glycerol throughout the >> purification to keep it soluble. I have been very worried that having >> glycerol in my protein solution when I am trying to crystallize it >> will >> prevent me from obtaining crystals. I am curious to see if others have >> successfully obtained crystals of proteins that required glycerol to >> keep >> them soluble and if so what concentration of glycerol could you use? >> Should I be concerned about this or am I worrying too much? >> Thanks for your input >> Rob >> -- Professor James Whisstock NHMRC Principal Research Fellow / Monash University Senior Logan fellow Department of Biochemistry and Molecular Biology Monash University, Clayton Campus, PO Box 13d, VIC, 3800, Australia +613 9905 3747 (Phone) +613 9905 4699 (Fax) +61 418 170 585 (Mobile)
