Dear All, I am working with a protein that requires 10% glycerol throughout the purification to keep it soluble. I have been very worried that having glycerol in my protein solution when I am trying to crystallize it will prevent me from obtaining crystals. I am curious to see if others have successfully obtained crystals of proteins that required glycerol to keep them soluble and if so what concentration of glycerol could you use? Should I be concerned about this or am I worrying too much? Thanks for your input Rob
-- Robert J.Gruninger B.Sc. PhD Student Department of Chemistry & Biochemistry University of Lethbridge, 4401 University Dr. Lethbridge, AB, Canada, T1K 3M4 Phone:(403)329-2746 Fax:(403)329-2057
