[Freesurfer] Overestimation of volume in Hippocampus

2017-02-27 Thread Michel Hu
Dear Freesurfer experts, I have run several subjects through the recon-all pipeline including the hippocampal segmentation with freesurfer 6.0 on Ubuntu 16.04. However when looking at the text file with the volumes it seems that all the numbers are bigger than normal as all my subjects have MCI

Re: [Freesurfer] Overestimation of volume in Hippocampus

2017-02-27 Thread Shane S
Hi Michel Hu, Is that a summed value of left and right hippocampus? That seems normal to me since averaged total hippocamppal volumes for MCI have been typically reported at around 2500-3500mm3. Thanks, --  Shane S On 27 February 2017 at 09:44:27, Michel Hu (a...@live.nl) wrote: Dear Freesurf

Re: [Freesurfer] Overestimation of volume in Hippocampus

2017-02-27 Thread Michel Hu
Dear Shane, I apologize for not being clear. The reported value is of only one half of the hippocampus. I have only run 4 subjects of the 75 however they all have a reported value of between 6000 to 1 mm^3 summed together for the hippocampus. Sincerely, Michel Hu _

Re: [Freesurfer] Overestimation of volume in Hippocampus

2017-02-27 Thread Shane S
Hi Michel Hu, Yes, that’s too large for MCI or even controls for that matter I think. Have you checked the aseg.mgz? I generally find that the hippocampus is well segmented, even in patients with a lot of atrophy. Are you taking the measurements with the asegstats2table? Sincerely, --  Shane

[Freesurfer] INTERSUBJECT AVERAGING

2017-02-27 Thread Marco Martinolli
Dear all, I am new to FreeSurfer and I want to reproduce on 20 subjects the registration strategy as explained in Fischl paper: High-resolution inter-subject averaging and a coordinate system for the cortical surface, Fischl, B., Sereno, M.I., Tootell, R.B.H., and Dale, A.M., (1999). Human Brain

Re: [Freesurfer] Overestimation of volume in Hippocampus

2017-02-27 Thread Michel Hu
Dear Shane, I have checked the aseg.mgz and in only one I would say the segmentation failed however the others look fine and I'm taking the measurements from the text file that is generated from the aseg.stats. Should I redo the recon-all with a different input? I have always given freesurfer T

Re: [Freesurfer] Overestimation of volume in Hippocampus

2017-02-27 Thread Antonin Skoch
Dear Michel, Given your resolution 0.8x0.8x0.8, did you run recon-all with -hires flag? What did you used T2 image for? As a -T2pial refinement? T2 image is not used for hippocampal segmentation in standard recon-all stream. But pay attention that with -hires you also have to alter default value

Re: [Freesurfer] Overestimation of volume in Hippocampus

2017-02-27 Thread Michel Hu
Dear Antonin, I did use the T2 scan for the T2pial refinement during the recon-all however I actually already preformed the hippocampal subfields and when i looked at the txt file generated from that the results are quite similar. I used the multispectral segmentation. I didn't not use the -hire

Re: [Freesurfer] Overestimation of volume in Hippocampus

2017-02-27 Thread Antonin Skoch
Dear Michel, by default in the recon-all the input images are resampled to 1x1x1mm3. To disable this and retain your resolution, you have to add -hires flag (and adapt the mris_inflate -n parameter). This could improve the segmentation results, however I would not expect that it is the reason

Re: [Freesurfer] Overestimation of volume in Hippocampus

2017-02-27 Thread Michel Hu
Dear Antonin, Thank you for the assistance! I really appreciate it. My final goal is to determine the volume of the dentate gyrus however I first wanted to establish if the volume of the whole hippocampus was within expected boundaries before moving on to the dentate gyrus. Do you perhaps know i

Re: [Freesurfer] Overestimation of volume in Hippocampus

2017-02-27 Thread Antonin Skoch
Dear  Michel, not sure if I understand. Do you mean that you cropped your T2 to FOV 256 mm ? I think that this should have no effect until you cropped parts of the brain. Maybe, as a part of quality-check you can check whether your T2 is correctly registered to T1 by inspection T1_to_.v10.QC.

Re: [Freesurfer] Overestimation of volume in Hippocampus

2017-02-27 Thread Michel Hu
Dear Antonin, I believe I figured out what I was doing wrong! I forgot to correct the voxel volumes with the intracranial volumes. After the correction I'm getting the expected values. I was referring to the -cw256 flag and i will check if the T2 is correctly registered to T1 Thanks again! Since

Re: [Freesurfer] Hippocampal Segmentation

2017-02-27 Thread Iglesias Gonzalez, Eugenio
Yes, it's the tail. All subfield are segmented jointly. Cheers E Sent from my phone, please excuse brevity and typos From: freesurfer-boun...@nmr.mgh.harvard.edu on behalf of Jessica Sevick Sent: Sunday, February 26, 2017 7:54:28 PM To: Freesurfer support list

Re: [Freesurfer] Fw: PETsurfer mri_coreg problem

2017-02-27 Thread miracle ozzoude
Hello Antonin and Doug, I viewed it in tkregister2 and I think the co-registration worked. Is there a way to extract info from both PET and anatomical to confirm the co-registration? Best, Paul On Sun, Feb 26, 2017 at 4:49 PM, Antonin Skoch wrote: > Dear Paul, > > I think that the transform win

[Freesurfer] Odd outputs from TRACULA

2017-02-27 Thread Magnús Ingvi
Hello! I have gone through the TRACULA tutorial, and the tracts are pretty odd. I'm wondering if I did something incorrectly, but as far as I can tell, I followed the tutorial step by step. The individuals in question are healthy, no irregularities in the brains. I have attached images of the fmajo

Re: [Freesurfer] Fw: PETsurfer mri_coreg problem

2017-02-27 Thread Douglas Greve
I'm not sure what you mean. If the registration looks good, what do you need to extract? On 2/27/17 9:03 AM, miracle ozzoude wrote: Hello Antonin and Doug, I viewed it in tkregister2 and I think the co-registration worked. Is there a way to extract info from both PET and anatomical to confir

Re: [Freesurfer] XL defect detected (with screenshots)

2017-02-27 Thread Douglas Greve
It is hard to say from those pics what is wrong as you need to look at the ?h.orig.nofix surface on the scans. It looks like there is a lot of skull left there, so it may be that the surface is extending out to the skull. I don't think it is a problem with the ventricles. On 2/26/17 6:58 AM,

Re: [Freesurfer] bbregister vs spmregister vs mri_coreg

2017-02-27 Thread Douglas Greve
I have not actually tested it on the mni152 reg. It might (probably does) work fine. On 2/25/17 5:33 PM, Antonin Skoch wrote: Dear Doug, I was wondering, why do you prefer mni152reg to mri_coreg for gregistration to MNI? According to help, mri_coreg is also able to do 12 DOF registration.

Re: [Freesurfer] bbregister vs spmregister vs mri_coreg

2017-02-27 Thread Douglas Greve
You can do the masking with mri_mask On 2/26/17 7:04 AM, Shane Schofield wrote: Dear Doug, If I want to create a new volume that is registered to the MNIspace (using mni1525reg), can I mask out other voxels that are not part of the mni template brain? It is a PET volume (already in individua

Re: [Freesurfer] Fw: PETsurfer mri_coreg problem

2017-02-27 Thread miracle ozzoude
Hello Doug, Thanks for replying. I meant like coordinate points on both images to compare (i.e., any metric different from visual inspection). Best, Paul. On Mon, Feb 27, 2017 at 10:50 AM, Douglas Greve wrote: > I'm not sure what you mean. If the registration looks good, what do you > need to ex

Re: [Freesurfer] make average subject

2017-02-27 Thread Douglas Greve
It should be created by recon-all. Check that subject to see whether it finished or had an error On 2/24/17 3:17 PM, Limachia, Gaurang (NIH/NINDS) [F] wrote: Hello Freesurfer Experts, I received this error while running the make_avg_subject script. I would greatly appreciate it if you can p

Re: [Freesurfer] INTERSUBJECT AVERAGING

2017-02-27 Thread Douglas Greve
Making a new folding atlas is not an easy task. I wrote a script to help called "make_folding_atlas". It is a very time consuming process, and the script assumes that you have access to a cluster running PBS/Torque through the pbsubmit command. If you don't have that, you could either write a

Re: [Freesurfer] Fw: PETsurfer mri_coreg problem

2017-02-27 Thread Douglas Greve
But what would you compare if you had these coordinates? What would the coordinates tell you? On 2/27/17 11:01 AM, miracle ozzoude wrote: Hello Doug, Thanks for replying. I meant like coordinate points on both images to compare (i.e., any metric different from visual inspection). Best, Paul.

[Freesurfer] QS for using cuda in FS6.0

2017-02-27 Thread 巍 魏
dear FS community: now i have many subjects may should rerun in FS6.0 so i thought could i use gpu for accelerating, and through searching in the mailing list i got some messages: 1. cuda was no longer supported 2. new features in cpu version were not add in gpu version as those above, is

Re: [Freesurfer] Strange wm error

2017-02-27 Thread Douglas Greve
Hi Mel, how does the orig or orig.nofix look? On 2/24/17 9:21 AM, Melanie Ganz wrote: Dear list, we are experiencing a strange wm surface error that we are not quite sure about how to fix. The errors is at a location where the wm.mgz look absolutely fine as well as the brainmask.mgz. Also th

Re: [Freesurfer] Fw: PETsurfer mri_coreg problem

2017-02-27 Thread miracle ozzoude
Hello Doug, Thanks for replying. It might help evaluate the accuracy of the co-registration. Usually, We use visual inspection (placing the crosshair on 1 image (either MRI or PET) and comparing the position of the crosshair in 1 image to another image). However, this is subjective and based on exp

[Freesurfer] chose between 5.3 and 6.0

2017-02-27 Thread ? ?
dear FS community: now i have more than 200 subjects which already finished recon-all with 5.3, since 6.0 released, i wonder should i re-run them with 6.0 as lots of changes like different surf-map and registration. i ran a bit of them as test and i found the pial problems were more than 5.3

Re: [Freesurfer] bbregister vs spmregister vs mri_coreg

2017-02-27 Thread Antonin Skoch
Dear Doug, thank you for the feedback. In this context I would like to ask you to share your opinion on the mri_cvs_register. This tool should be far the most accurate for the registration to MNI, than any of affine-based registration tools, shouldn't be? Are there any pitfalls/disadvantages (a

Re: [Freesurfer] Fw: PETsurfer mri_coreg problem

2017-02-27 Thread Douglas N Greve
I don't think there is a better way than visual inspection. You could look at the final cost value, but that is relative, ie, you could get a bunch of subjects who you know have a good registration and create a table of the final costs, then when you get a new subject, compute the z-score of th

Re: [Freesurfer] bbregister vs spmregister vs mri_coreg

2017-02-27 Thread Douglas N Greve
Hi Antonin, mni152reg was just meant to replicate the affine registration used by other software and to provide users with a fast-and-easy way to do volume-based (subcortical) analysis in FS. I would prefer to use CVS for everything, but, as you mention, it does have a huge computational footp

Re: [Freesurfer] Intensity information of Hippocampal Subfields

2017-02-27 Thread ali reza mohammadi nejad
Thank you so much Dr. Doug! I just have one more question: Now I want to compute the same information for our second modality (i.e., mean and std intensity of different HP_subfields for our additional scan, e,g., T2 or FLAIR). My question is that how could I use .FSspace.mgz (in mri folder) and

Re: [Freesurfer] Intensity information of Hippocampal Subfields

2017-02-27 Thread Douglas N Greve
There is usually a T2.norm.mgz file in the mri folder. If that is there, then you should use that rather than the raw data that is not bias field corrected On 02/27/2017 12:30 PM, ali reza mohammadi nejad wrote: > Thank you so much Dr. Doug! I just have one more question: > > Now I want to comp

Re: [Freesurfer] Intensity information of Hippocampal Subfields

2017-02-27 Thread ali reza mohammadi nejad
I processed the T1-weighted data without T2. Therefore, we do not have T2.norm.mgz in the mri folder of each subject. Only for the HP-subfields segmentation, we added additional high-resolution T2-weighted data to increase the accuracy of HP-subfields segmentation. Thanks! Alireza > On Feb 27,

Re: [Freesurfer] Intensity information of Hippocampal Subfields

2017-02-27 Thread Douglas N Greve
Is there a T2.prenorm.mgz in the mri folder? If not, you can run bbregister --mov T2.nativespace.mgz --t2 --s subject --reg t2.reg.lta mri_vol2vol --mov T2.nativespace.mgz --reg t2.reg.lta --targ orig.mgz --o T2.prenorm.mgz Then run something like mri_normalize \ -sigma 0.5 -nonmax_su

[Freesurfer] Manual edit to include missing cortex?

2017-02-27 Thread Rockers, Elijah D.
Is there something I can do to extend the pial surface to include cortex that it ignored? In some places there are rather large chunks of cortex not included in the pial surface. http://imgur.com/a/FnAgj Link is to a screenshot, notice the top of the brain. It's fairly pronounced, visible in 12 a

Re: [Freesurfer] Manual edit to include missing cortex?

2017-02-27 Thread Lee Tirrell
Dear Eli, You may have to draw in voxels in the wm.mgz volume if it doesn't extend out far enough. After rerunning, this will push out the white surface, which causes the pial surface to be positioned further out. You can refer to our tutorial page for more information: https://surfer.nmr.m

[Freesurfer] How to output particular area from stats subdir

2017-02-27 Thread Jing Gu
Dear FreeSurfer Developers, By using the following code, we can get volume data of different areas from stats file. asegstats2table --subjects N01 --meas volume --tablefile aseg_stats.txt How can I get information of one or two areas, such as hippocampal? thanks Best, Gina ___

Re: [Freesurfer] chose between 5.3 and 6.0

2017-02-27 Thread Bruce Fischl
Hi weiwei if the aseg looks good you don't have to worry about how the subcortical structures look in the brainmask - it's irrelevant. Not sure about the pial surfaces - we find the surfaces in 6.0 more accurate than 5.3, but maybe not for your specific sequence? It's hard to say cheers Bruc

[Freesurfer] Ct file

2017-02-27 Thread yusif Al-kheder
Hello, Is there a way to use freesurfer to get coordinates and volume information from a ct file? Youssif ___ Freesurfer mailing list Freesurfer@nmr.mgh.harvard.edu https://mail.nmr.mgh.harvard.edu/mailman/listinfo/freesurfer The information in this

Re: [Freesurfer] Ct file

2017-02-27 Thread Douglas N Greve
you can register the CT to the T1 with bbregister On 02/27/2017 07:17 PM, yusif Al-kheder wrote: > Hello, > Is there a way to use freesurfer to get coordinates and volume information > from a ct file? > > Youssif > ___ > Freesurfer mailing list > Frees

[Freesurfer] Different segmentation atlas

2017-02-27 Thread Julie Hall
Dear all, I would like to use the Gordon Parcellation as segmentation atlas for my subjects. I have warped this atlas into individual subject space. Then I wanted to use mris_sample_parc to apply this parcallation, but I have a few questions.. 1. Is this the right command to use? 2. When

Re: [Freesurfer] gzip: stdin: unexpected end of file

2017-02-27 Thread Kambiz Tavabi
sha1sum freesurfer-Linux-centos6_x86_64-stable-pub-v6.0.0.tar.gz returns da39a3ee5e6b4b0d3255bfef95601890afd80709 A corrupted download three times! Do I just try downloading again? On Thu, Feb 23, 2017 at 9:02 AM wrote: > Send Freesurfer mailing list submissions to > freesurfer@nmr.mgh.

Re: [Freesurfer] gzip: stdin: unexpected end of file

2017-02-27 Thread zkaufman
I just did a test download on my home network and I got the correct sha 1723ef916c4e74ee8fff72438e9c6c91c79bd4cb freesurfer-Linux-centos6_x86_64-stable-pub-v6.0.0.tar.gz Perhaps your network times out after a certain time period, or has a cap on the file size? > sha1sum freesurfer-Linux-cent

[Freesurfer] Building Freesurfer dev with GPU support/CUDA enabled

2017-02-27 Thread Francis Tyson Thomas
Hi, I have been trying to build the freesurfer dev version with GPU support as per the information I had from my previous try a couple of months ago that Richard help me to successfully complete and I have not been successful in that. I followed the instructions from https://surfer.nmr.mgh.harvar

[Freesurfer] ACPC alignment before or after running on Freesurfer?

2017-02-27 Thread Lee Subin Kristine
Dear Freesurfer team, This may sound like a bit of a small question, but I could not find any exact answer elsewhere. Does the exact orientation of the input image have much effect on the accuracy of the segmentation in Freesurfer? It seems that some people apply ACPC alignment to the output of