Dear all,
Please see the job advert for an EM programmer below.
All responses/inquires to: sche...@mrc-lmb.cam.ac.uk
---
At the MRC Laboratory of Molecular Biology in Cambridge we are looking
to recruit a permanent Programmer to be respons
Dear all,
We have a postdoctoral position available for a talented and highly
motivated individual to undertake single molecule microscopy studies on
bacterial DNA replication and DNA repair. The lab focuses on the
structural aspects of DNA replication and translesion synthesis DNA
repair, us
Dear all,
I can get large amounts of a protein that is purified from inclusion
bodies. The protein is solubilized using 6M urea and refolded by dialysis.
However, treatment with urea is known to modify proteins
(N-term/Lys/Arg), which could ultimately effect crystallization.
Is this somethi
Hi Engin,
I have seen the same with HKL2000, especially with weak diffracting
crystals.
Don't know if this is correct, but another way to overcome this is
to lower the value of "error density" during integration.
I've seen the value for error density vary between different sites
(beamlin
informal inquiries please contact Dr Meindert Lamers:
mlam...@mrc-lmb.cam.ac.uk or visit
http://www2.mrc-lmb.cam.ac.uk/groups/mlamers/.
Applications must be made online at http://jobs.mrc.ac.uk using the
reference number: LMB09/549. If you do not have internet access or
experience technical
How about DNA synthesis within crystals?
1: Kiefer JR, Mao C, Braman JC, Beese LS. Visualizing DNA replication in
a catalytically active Bacillus DNA polymerase crystal. Nature. 1998 Jan
15;391(6664):304-7. PubMed PMID: 9440698.
2: Johnson SJ, Taylor JS, Beese LS. Processive DNA synthesis obs
you there,
Meindert Lamers
--
**
Meindert H. Lamers
Medical Research Council
Laboratory of Molecular Biology
Hills Road,
Cambridge, CB2 0QH
United Kingdom
tel +44 (0)1223 402401
fax +44 (0)1223 213556
web: http://www2.mrc-lmb.cam.ac.uk/groups/mlamers/
**
Dear all,
Is there any convention on the numbering of residues in a fusion protein?
I have a structure of two domains fused together but would like to keep
the biological numbering intact.
1st domain: residue 200-300 (protein A).
2nd domain: residue 170-350 (protein B).
The fusion is between A
: From Genome to
Proteome, 11 Dec 2012
Date: Thu, 11 Oct 2012 15:57:56 +0100
From: Meindert Lamers
To: CCP4BB@JISCMAIL.AC.UK
Dear all,
We are happy to announce the 2012 BCA Biological Structures Group winter
meeting “From Genome to Proteome”.
The meeting will take place on Dec 11 2012 at the LMB
