Yarrow,
as far as I know, scalepack, scalepackvirus and the other variants only
differ by their respective array sizes (Someone from Wladek's or ZO's
lab may be more suitable to comment on this). The rejections in the log
file are controlled by the parameters 'write rejection file xxx' and
'rejec
Staurosporine come to mind as a general kinase inhibitor. I also second
Artem's suggestion that ligands make a big difference, we had several
cases of kinases which required ligands for crystallization success.
Also make sure you eliminate any floppy ends which may interfere with
packing.
Good
Wenhe,
Bioedit (http://www.mbio.ncsu.edu/bioedit/bioedit.html) is able to do
this. You can also export a nice alignment as RTF file into Word from
the graphics view. This comes in handy for publication and presentation.
Cheers,
Carsten
From: CCP4 bulletin board [ma
John,
You can use APBS in conjunction with PDB2PQR and PROPKA (all available
from the ABPS website) for the calculations and Pymol for visualization.
Good luck
Carsten
-Original Message-
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of
john peter
Sent: Friday, Augus
Hi Louise,
for internal use the name of your ligand does not matter as long as you have a
.cif file describing the restraints. I am not sure about the behavior of
refmac, but in phenix the presence of a defined name in a .cif file takes
precedence over any duplicate entry in your monomer librar
Hi Allen,
MR with low homology search models is also sensitive to the quality of your
dataset. Besides the other recommendations already offered make sure that your
dataset is of high quality. I recommend collecting a couple of good natives and
try them all in your search.
Good luck
Tarun,
in my group we used to have a Proteum6000 CCD from Bruker. With the right
detector descriptions from the HKL folks we able to use HKL2000, talk to the
hkl folks, you may also need another license covering that detector though.
Alternatively Bruker provides the Proteum suite (I think that
Phenix has a menu item for this: Reflection Tools -> Import CIF structure
factors
HTH
Carsten
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of StrBio
Sent: Monday, June 15, 2015 1:53 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] workable data from PDB sf-ci
Hi Tim,
with regards to the point you and others raised with regards to funding
provided by industry users:
From my point of view as a Structural Biology group leader responsible for
crystallographic software budget decisions in a major Pharma franchise, I very
much appreciate the effort and
Hi Francois,
instead of addressing the problem from the PDB side, you could take a look at
the "embed" feature in Pymol. It allows embedding structural information into a
special command script. It ties you down to PyMol, but on the other hand you
don't need to worry about extending the PDB for
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