Staurosporine come to mind as a general kinase inhibitor. I also second
Artem's suggestion that ligands make a big difference, we had several
cases of kinases which required ligands for crystallization success.
Also make sure you eliminate any floppy ends which may interfere with
packing.
Good luck
Carsten
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of
CHAVES SANJUAN, ANTONIO
Sent: Monday, January 30, 2012 5:07 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Kinase crystallization
Dear all,
I am trying to crystallize a protein kinase without any success.
I suspect about its characteristic catalytic loop. I have already
prepared different constructs, different expression vectors, and
different mutant proteins (pseudo-phosphorylated, active, inactive?). I
have also tested some ligands as ANPpnp (non hidrolizable nucleotide),
ADP (product) and manganese (cofactor).
I am thinking in trying to cocrystallize it with a general kinase
substrate (a peptide, a small molecule...). Does any one have any
experience or suggestion?
Thanks in advance.
Sincerely,
Antonio
