Hi Allen, MR with low homology search models is also sensitive to the quality of your dataset. Besides the other recommendations already offered make sure that your dataset is of high quality. I recommend collecting a couple of good natives and try them all in your search.
Good luck Carsten From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of allen price Sent: Tuesday, January 06, 2015 7:06 AM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] Some advices on model modification Dear all: I got a dataset at 2.8 angstron. I have tried several ways such as phaser, MRBUMP,BALBES,but still can't solve the data,which means I have to edit my model. Maybe I'd better cut it off or delete the water or loop. I really have no idea,as it is my first time to do such things, I alway used the whole model to mr. Could anyone give me some advice? what kind of software do you guys use? really need you help! Best regards, Allen