Wenhe,
Bioedit (http://www.mbio.ncsu.edu/bioedit/bioedit.html) is able to do this. You can also export a nice alignment as RTF file into Word from the graphics view. This comes in handy for publication and presentation. Cheers, Carsten From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of WENHE ZHONG Sent: Friday, February 03, 2012 12:32 AM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] off-topic: "special" format for multiple sequence (protein) alignment Dear members, Apologize for this off-topic question. I am looking for a protein sequence alignment tool which is capable to generate a particular output file similar to the attached format (please see the attached picture). I have been looking at some popular programs but none of them can show the conserved amino acids by colored blocks as shown in the attached file. Maybe some of you have seen some programs can do this? Thank you. King regards, Wenhe