Hi Eric,
If you are trying to express your Se-Met protein, it is typically done in a
defined media starting with a minimal media base so a mixture of amino acids,
particularly those that are essential, is needed. You likely will not get very
high yields of SeMet-labeled protein if you simply
Hi folks,
I am currently impressed by the efficiency of dehydration treatments over
the diffraction capacity of our crystals in one particular condition.
Without any treatment the crystals seldom diffract to 20-30A but in our last
synchrotron trip the very same crystals, after been incubated with
Hi Israel!
The phenomena you describe seems to be related to cryoprotection, but
you don't say anything about collection temperature or cryoprotection
used. Controlled-slow dehydration is one way of achieving optimized
cryocooling of specially fragile crystals. I would recommend you to
re
Dear Israel,
There is a lot to look up in this field. I believe it started with
Schick, B. & Jurnak, F. (1994). Acta Cryst. D50, 563-568.
at http://scripts.iucr.org/cgi-bin/paper?gr0289
There is a nice review online at
http://www-bio3d-igbmc.u-strasbg.fr/~
Dear Israel,
there is also a dedicated humidity device developed at EMBL/ESRF
Grenoble that allows for systematic dehydration experiments to be
carried out at room temperature and soon at 4°C. Here is the primary
reference:
Sanchez-Weatherby, J., Bowler, M.W., Huet, J., Gobbo, A., Felisaz, F
Hi Israel,
Just to add to that, and as I know you use the Diamond synchrotron, the
device mentioned is available (on advance request) on the beamline I02.
Cheers
-- David
On 1 May 2011 19:50, Martin Weik wrote:
> Dear Israel,
>
> there is also a dedicated humidity device developed at EMBL/ES
Dear Israel,
This paper describes a procedure to crystal desiccation that allowed the
improvement of diffraction: C. Abergel Acta Cryst. (2004). D60, 1413-1416
http://scripts.iucr.org/cgi-bin/paper?S0907444904013678
HTH
Good luck,
Catarina
--
Catarina Rodrigues, PhD Student
Molecular Transp
Dear Israel,
as Martin pointed out we have a device here at the ESRF/EMBL, the
HC1b, that produces a stream of air with a precisely controlled RH at
the sample position that we have used with some success to monitor the
effects dehydration has on diffraction quality. The same device is al
