Hi Israel!
The phenomena you describe seems to be related to cryoprotection, but
you don't say anything about collection temperature or cryoprotection
used. Controlled-slow dehydration is one way of achieving optimized
cryocooling of specially fragile crystals. I would recommend you to
read Garman's works, particularly the one that follows:
Cryocooling of macromolecular crystals: optimization methods
EF Garman? - Methods in enzymology, 2003
Enjoy
Horacio
Quoting Israel Sanchez <israelsan...@gmail.com>:
Hi folks,
I am currently impressed by the efficiency of dehydration treatments over
the diffraction capacity of our crystals in one particular condition.
Without any treatment the crystals seldom diffract to 20-30A but in our last
synchrotron trip the very same crystals, after been incubated with
increasing concentration of low molecular weight PEGs diffracted to 6A.
I was wondering if anyone has studied these effects in a systematic way.
Does anyone on the ccp4bb knows references or has any
experience/pseudo-religious believes that do not care to share with the
community about this particular topic?
Thank you very much in advance!!!!
--
Israel Sanchez Fernandez PhD
Ramakrishnan-lab
MRC Laboratory of Molecular Biology,
Hills Road, Cambridge, CB2 0QH, UK
