Dear Israel, This paper describes a procedure to crystal desiccation that allowed the improvement of diffraction: C. Abergel Acta Cryst. (2004). D60, 1413-1416
http://scripts.iucr.org/cgi-bin/paper?S0907444904013678 HTH Good luck, Catarina -- Catarina Rodrigues, PhD Student Molecular Transport & Signalling AFMB UMR 6098 CNRS/UI/UII Case 932 163 Avenue de Luminy 13288 Marseille cedex 9 (France) Tel : +33 04 91 82 55 60 Fax : 04 91 26 67 20 e-mail:catarina.rodrig...@afmb.univ-mrs.fr http://www.afmb.univ-mrs.fr/ Le 1 mai 2011 à 19:32, Israel Sanchez a écrit : > Hi folks, > > > I am currently impressed by the efficiency of dehydration treatments over the > diffraction capacity of our crystals in one particular condition. Without any > treatment the crystals seldom diffract to 20-30A but in our last synchrotron > trip the very same crystals, after been incubated with increasing > concentration of low molecular weight PEGs diffracted to 6A. > > I was wondering if anyone has studied these effects in a systematic way. Does > anyone on the ccp4bb knows references or has any experience/pseudo-religious > believes that do not care to share with the community about this particular > topic? > > > Thank you very much in advance!!!! > > > -- > Israel Sanchez Fernandez PhD > Ramakrishnan-lab > MRC Laboratory of Molecular Biology, > Hills Road, Cambridge, CB2 0QH, UK > > > > -- > This message has been scanned for viruses and > dangerous content by MailScanner, and is > believed to be clean. -- This message has been scanned for viruses and dangerous content by MailScanner, and is believed to be clean.
