Dear Dr. Thomas
It's not SAD/MAD data I have solved it using molrep only.
I will try alternate positions for met and see the density again.
Thanx to all for valuable suggestions
Ethayathulla
On Fri, 13 Apr 2007 Thomas Edwards wrote :
>
>Dear Ethayathulla ,
>
>did you solve the structure b
I think the SD-CE is pointing the wrong way. And they look much too close.
I would rotate them and re-refine
Eleanor
PS - any noise on the 3 fold axis will be multiplied by a factor of 3
Ethayathulla Abdulsamath wrote:
Dear all
I am doing one structure at 2.6A resolution where I found unu
This appears to be a symmetry threefold axis. These areas are notoriously
prone to have density artefacts. Also it looks like your terminal carbons
are pointing in the opposite way, and the entire end of Met is slightly
shifted as a result. You may never be able to fit this area to your
satisfactio
Dear Ethayathulla ,
did you solve the structure by SeMet MAD or some other way?
Sometimes you get strange densities around the ends of methionine side
chains (around the S/Se) when switching from SeMet data to native.
Cheers
Ed
__
T.Edwards Ph.D.
Astbury Centre for Structural
When I experienced strong difference density near methionines, It could be
explained by an alternative conformation of the residue...
greetings, david
Original-Nachricht
Datum: Fri, 13 Apr 2007 09:51:09 -
Von: Ethayathulla Abdulsamath <[EMAIL PROTECTED]>
An: [EMAIL PROTECT
