I think the SD-CE is pointing the wrong way. And they look much too close.
I would rotate them and re-refine
Eleanor
PS - any noise on the 3 fold axis will be multiplied by a factor of 3
Ethayathulla Abdulsamath wrote:
Dear all
I am doing one structure at 2.6A resolution where I found unusual
density near methionines. Actually three methionines come close
together nearby and I get difference even at 5sigma cutoff. I don't
understand nature of the density. The amino acid sequnce is same I
mean met by chemical sequncing so there is no change amino acid
residues. I am sending the snapshot of the difference fourier observed
at methionines. The crystal belong to R32 system with one molecule and
biological trimer. In snapshot two of the methionines are symmetry
related.
Details of the data collection.
Data collected at synchrotron
Overall Rsym = 6 % and I/sigma = 2.1. Completeness = 99%
Can anyone suggestion what could be chemical nature for the difference
fourier.
Thanks in advance
Ethayathulla
###################################################
A.S.Ethayathulla,Ph.D.
Department of Biophysics
All India Institute of Medical Sciences
Ansari Nagar
New Delhi-110029
India.
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