When I experienced strong difference density near methionines, It could be explained by an alternative conformation of the residue...
greetings, david -------- Original-Nachricht -------- Datum: Fri, 13 Apr 2007 09:51:09 -0000 Von: Ethayathulla Abdulsamath <[EMAIL PROTECTED]> An: [EMAIL PROTECTED] Betreff: [ccp4bb] Unusual Difference Fourier near Methionines > > Dear all > > I am doing one structure at 2.6A resolution where I found unusual density > near methionines. Actually three methionines come close together nearby and > I get difference even at 5sigma cutoff. I don't understand nature of the > density. The amino acid sequnce is same I mean met by chemical sequncing so > there is no change amino acid residues. I am sending the snapshot of the > difference fourier observed at methionines. The crystal belong to R32 system > with one molecule and biological trimer. In snapshot two of the methionines > are symmetry related. > > Details of the data collection. > Data collected at synchrotron > > Overall Rsym = 6 % and I/sigma = 2.1. Completeness = 99% > > Can anyone suggestion what could be chemical nature for the difference > fourier. > > Thanks in advance > > Ethayathulla > > ################################################### > A.S.Ethayathulla,Ph.D. > Department of Biophysics > All India Institute of Medical Sciences > Ansari Nagar > New Delhi-110029 > India. > ################################################### -- "Feel free" - 10 GB Mailbox, 100 FreeSMS/Monat ... Jetzt GMX TopMail testen: http://www.gmx.net/de/go/topmail
