As a follow-up, we have a postdoc position open to further develop metal
identification methods for crystals and in solution!
The project: structural study of biological systems that are impacted by free
radical activity and metalloproteins, where the modeled metal and/or
coordination state are
Dear Andy,
We can easily reach the Ca absorption edge on I23 (Diamond long wavelength
beamline), so we can tell you if you have calcium in your crystal but also its
location.
Have a look at the beamline webpage:
https://www.diamond.ac.uk/Instruments/Mx/I23.html
Cheers,
Kamel
Hi Andy,
We were able to use EDX fluorescence scans at a beam line to detect calcium in
crystals.
https://pubmed.ncbi.nlm.nih.gov/29890074/
It isn't quantitative like micro-pixe but you can see it...
Eddie Snell and Elspeth Garman and team have done some further work on
micro-pixe, as well –
Hi Andrew,
We had considerable success with MicroPIXE collaborating with the Garman group
in Oxford and the Surrey Ion Beam Center. There is a tiny amount of sample
needed, it's an atomic technique so the sample can be from that old tube that
hadn't been discarded yet, and it provides identific
Hello again, re: amount, yes, from memory, it was a few milligrams. We also
used EDAX on dried samples IIRC. Cheers, Jon.C.
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Original Message
On 7 Jan 2021, 14:13, Andrew Lovering wrote:
> Dear CCP4ers
>
> We have a protein with moderate resolution (
Hello, there is atomic-absorption. Breaks your heart to see the protein going
into the flame, but we got good results for Se and transition metals with a
superoxide dismutase. I doubt if there'll ever be one of those on a beam line
;-0 Cheers, Jon Cooper.
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