Hello again, re: amount, yes, from memory, it was a few milligrams. We also used EDAX on dried samples IIRC. Cheers, Jon.C.
Sent from ProtonMail mobile -------- Original Message -------- On 7 Jan 2021, 14:13, Andrew Lovering wrote: > Dear CCP4ers > > We have a protein with moderate resolution (mid 2s) with obvious metal ion > density holding the oligomer together. We can make an educated guess that the > ions are likely to be Mg/Ca and can look at co-ordination and bond > lengths…..but I have a more specific question – would it be appropriate to > try and identify these at the beamline? Obviously the edges for these aren’t > accessible to sit either side of the peak but perhaps something like > micro-pixe would be fun; is this done routinely? > > https://doi.org/10.1016/j.pbiomolbio.2004.09.005 > > Best & thanks in advance > > Andy > > --------------------------------------------------------------- > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
