Dear Andy, We can easily reach the Ca absorption edge on I23 (Diamond long wavelength beamline), so we can tell you if you have calcium in your crystal but also its location. Have a look at the beamline webpage: https://www.diamond.ac.uk/Instruments/Mx/I23.html Cheers, Kamel ________________________________ From: CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK> on behalf of Andrew Lovering <a.lover...@bham.ac.uk> Sent: 07 January 2021 14:13 To: CCP4BB@JISCMAIL.AC.UK <CCP4BB@JISCMAIL.AC.UK> Subject: [ccp4bb] Elemental Analysis
Dear CCP4ers We have a protein with moderate resolution (mid 2s) with obvious metal ion density holding the oligomer together. We can make an educated guess that the ions are likely to be Mg/Ca and can look at co-ordination and bond lengths…..but I have a more specific question – would it be appropriate to try and identify these at the beamline? Obviously the edges for these aren’t accessible to sit either side of the peak but perhaps something like micro-pixe would be fun; is this done routinely? https://doi.org/10.1016/j.pbiomolbio.2004.09.005 Best & thanks in advance Andy ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 -- This e-mail and any attachments may contain confidential, copyright and or privileged material, and are for the use of the intended addressee only. If you are not the intended addressee or an authorised recipient of the addressee please notify us of receipt by returning the e-mail and do not use, copy, retain, distribute or disclose the information in or attached to the e-mail. Any opinions expressed within this e-mail are those of the individual and not necessarily of Diamond Light Source Ltd. Diamond Light Source Ltd. cannot guarantee that this e-mail or any attachments are free from viruses and we cannot accept liability for any damage which you may sustain as a result of software viruses which may be transmitted in or with the message. Diamond Light Source Limited (company no. 4375679). Registered in England and Wales with its registered office at Diamond House, Harwell Science and Innovation Campus, Didcot, Oxfordshire, OX11 0DE, United Kingdom ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
