Re: [ccp4bb] recommendation for heavy atoms used in lithium sulfate and ammonium sulfate

2007-04-21 Thread Tommi Kajander
gt; "Open your eyes. You have only to see things clearly, to understand." > -Leonardo da Vinci > > -----Original Message----- > From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf Of > Tiancen Hu > Sent: Thursday, April 19, 2007

Re: [ccp4bb] recommendation for heavy atoms used in lithium sulfate and ammonium sulfate

2007-04-20 Thread artem
Tiancen If you have enough crystals to experiment with, just do a normal series of heavy atom derivatives, starting with good old favorites such as mercury and platinum. As an added touch of class you can try tungstate - it's like sulphate, but has fat W so it can be used probably just like a hali

Re: [ccp4bb] recommendation for heavy atoms used in lithium sulfate and ammonium sulfate

2007-04-20 Thread Jeffrey . Kieft
stand." -Leonardo da Vinci -Original Message- From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf Of Tiancen Hu Sent: Thursday, April 19, 2007 10:27 PM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] recommendation for heavy atoms used in lithium sulfate an

Re: [ccp4bb] recommendation for heavy atoms used in lithium sulfate and ammonium sulfate

2007-04-20 Thread Ronnie Berntsson
Hi Tiancen, I can recommend using a Halide soak (bromide or iodine). You could try to "exchange" part your salt with sodium bromide. Always use a bad looking crystal for the first soak though to see that it doesn't get damaged by the soak. See for further reference: Novel approach to phas

[ccp4bb] recommendation for heavy atoms used in lithium sulfate and ammonium sulfate

2007-04-19 Thread Tiancen Hu
Dear all, Could any one recommend some heavy atoms used for crystals grown in 0.1M tri-sodium citrate pH 5.6, 1M Lithium sulfate and 0.5M ammonium sulfate? I read from Hampton user guide of heavy atom kit that "high salt concentrations are not the ideal medium for heavy atom reactions with mac