Dear all, Could any one recommend some heavy atoms used for crystals grown in 0.1M tri-sodium citrate pH 5.6, 1M Lithium sulfate and 0.5M ammonium sulfate? I read from Hampton user guide of heavy atom kit that "high salt concentrations are not the ideal medium for heavy atom reactions with macromolecules", so is there any type of heavy atom we should avoid using? We do not have any experience in preparing heavy atom derivative, so any suggestions, experience or references will be greatly appreciated.
Thanks in advance! Tiancen Hu Shanghai Institute of Materia Medica
