Tiancen If you have enough crystals to experiment with, just do a normal series of heavy atom derivatives, starting with good old favorites such as mercury and platinum. As an added touch of class you can try tungstate - it's like sulphate, but has fat W so it can be used probably just like a halide soak...
Let me know if you need more detailed help :) Artem > Dear all, > > Could any one recommend some heavy atoms used for crystals grown in 0.1M > tri-sodium citrate pH 5.6, 1M Lithium sulfate and 0.5M ammonium sulfate? I > read from Hampton user guide of heavy atom kit that "high salt > concentrations are not the ideal medium for heavy atom reactions with > macromolecules", so is there any type of heavy atom we should avoid using? > We do not have any experience in preparing heavy atom derivative, so any > suggestions, experience or references will be greatly appreciated. > > Thanks in advance! > > Tiancen Hu > Shanghai Institute of Materia Medica >
