You can also try products from Bio-rad.
http://www.bio-rad.com/featured/en/nickel-columns-nickel-resin.html
Best!
--
卢作焜
南开大学新生物站A202
Lu Zuokun, Ph.D. Candidate
College of Life Science, Nankai University
At 2017-02-18 04:31:08, "Kevin Jin" wrote:
Sorry, the company was CloneTech, now
Sorry, the company was CloneTech, now it is Takara (not Takeda). You may
talk with Dr. Gia Jokhadze, who is the director of protein chemistry
department. He would give you more information. You can find his profile
on linkedin. He is very nice person. Probably, you can ask some free sample
from hi
Probably you can try His-Column from Clonetech (Takeda ?). They modified
the column packing using nylon membrane, which offered better flow-through
than that of traditional resin-gel column.
On Wed, Feb 15, 2017 at 8:57 AM, Markus Seeliger
wrote:
> Dear all,
> we are happy users of all that GE
We used cOmplete Histag column from Roche, 5 ml prepacked. We bought 3-4
prepacked columns and a bottle of 25 ml (cheaper). The nice thing about
this Roche pack, if you closed the outlet and let the pump run into the
inlet, the cap will slowly detach .. We use this trick to replace the
resin in
I'll second those suggestions and also note that empty XK columns from GE
regularly show up on eBay for pennies on the dollar.
Roch cOmplete is really great in situations where there are chelators
around (especially proteins secreted into eukaryotic tissue culture media).
It saved me tremendous am
We have had good luck with columns from Essential Life Solutions (
http://www.essential-life.net/). They are easy to work with and hold up
under pressure well.
Best,
David
--
David L. Blum, Ph.D.
Director, Bioexpression and Fermentation Facility
Department of Biochemistry and Molecular Biol
Well GE sells empty columns (XK for low back-pressure, tricorn for higher
back-pressure) that you can fill with the resin you want. They seem
expensive but for that kind of things I don't think any option will be real
'savings'. For nickel affinity we have been having good results with
Roche's Comp
Hi Markus,
BioRad has columns as well (prepacked and empty), which follow the
establiched connector standard. So usable without the need of adapters.
Cheers
Christian
Am 15.02.2017 um 16:57 schrieb Markus Seeliger:
Dear all,
we are happy users of all that GE offers around their FPLC system
MD 21201
Tel: (410) 706-7457
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Markus
Seeliger
Sent: Wednesday, February 15, 2017 11:57 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] off topic: Alternatives to GE HiTrap columns
Dear all,
we are happy users of all that GE
Dear all,
we are happy users of all that GE offers around their FPLC system, but I am
getting a little tired of feeling monopolized. Are any of you aware of
either empty columns or prepacked columns (e.g. metal affinity or ion
exchange resins) from other companies?
Thanks for your advice
Markus
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