Well GE sells empty columns (XK for low back-pressure, tricorn for higher back-pressure) that you can fill with the resin you want. They seem expensive but for that kind of things I don't think any option will be real 'savings'. For nickel affinity we have been having good results with Roche's Complete - it requires less imidazole for binding and elution, and at the same time it is compatible with reducing agents and chelators. And we still buy the HisTrap FF crude.
On Wed, Feb 15, 2017 at 6:03 PM, Christian Roth <christianroth...@gmail.com> wrote: > Hi Markus, > > BioRad has columns as well (prepacked and empty), which follow the > establiched connector standard. So usable without the need of adapters. > > Cheers > > Christian > > Am 15.02.2017 um 16:57 schrieb Markus Seeliger: > > Dear all, > we are happy users of all that GE offers around their FPLC system, but I > am getting a little tired of feeling monopolized. Are any of you aware of > either empty columns or prepacked columns (e.g. metal affinity or ion > exchange resins) from other companies? > Thanks for your advice > > Markus > > >
