You could try Goldbio.com. I have not used the His-columns from them, but have used the Protein A columns and they work as well as the Protein A from GE.
Daniel A Bonsor PhD. Sundberg Lab Institute of Human Virology University of Maryland, Baltimore 725 W Lombard Street N370 Baltimore Maryland MD 21201 Tel: (410) 706-7457 From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Markus Seeliger Sent: Wednesday, February 15, 2017 11:57 AM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] off topic: Alternatives to GE HiTrap columns Dear all, we are happy users of all that GE offers around their FPLC system, but I am getting a little tired of feeling monopolized. Are any of you aware of either empty columns or prepacked columns (e.g. metal affinity or ion exchange resins) from other companies? Thanks for your advice Markus