I'll second those suggestions and also note that empty XK columns from GE regularly show up on eBay for pennies on the dollar.
Roch cOmplete is really great in situations where there are chelators around (especially proteins secreted into eukaryotic tissue culture media). It saved me tremendous amounts of time in one case, where I no longer had to concentrate/dialyze liters worth of media but could just filter it and shoot it through a column. -Tom ----------------------------- Thomas Cleveland, Ph.D. NIST Center for Neutron Research 100 Bureau Dr. MS 6102 Gaithersburg, MD 20899-6102 On Wed, Feb 15, 2017 at 1:04 PM, Carlos Kikuti <kik...@gmail.com> wrote: > Well GE sells empty columns (XK for low back-pressure, tricorn for higher > back-pressure) that you can fill with the resin you want. They seem > expensive but for that kind of things I don't think any option will be real > 'savings'. For nickel affinity we have been having good results with > Roche's Complete - it requires less imidazole for binding and elution, and > at the same time it is compatible with reducing agents and chelators. And > we still buy the HisTrap FF crude. > > On Wed, Feb 15, 2017 at 6:03 PM, Christian Roth < > christianroth...@gmail.com> wrote: > >> Hi Markus, >> >> BioRad has columns as well (prepacked and empty), which follow the >> establiched connector standard. So usable without the need of adapters. >> >> Cheers >> >> Christian >> >> Am 15.02.2017 um 16:57 schrieb Markus Seeliger: >> >> Dear all, >> we are happy users of all that GE offers around their FPLC system, but I >> am getting a little tired of feeling monopolized. Are any of you aware of >> either empty columns or prepacked columns (e.g. metal affinity or ion >> exchange resins) from other companies? >> Thanks for your advice >> >> Markus >> >> >> >
