iation characteristics.
5. There are papers on capillary electrophoresis methods where they
study exactly these effects (time of equilibrium vs time of
separation).
A.
Maia
- Original Message -
From: "aidong"
To:
Sent: Sunday, July 04, 2010 3:01 AM
Subject: Re: [cc
Hi,
'The Role of Diffusion in Enzyme Kinetics'
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1367934/pdf/biophysj00672-0040.pdf
Mark
~
Dr Mark Agacan, Scientific Officer for the Division of Biological Chemistry and
Drug Discovery,
Wel
Hi Jacob -
Sorry if I caused any misunderstanding, but I am not saying that kD is
not mattering in cells.
Of course it should matter.
I am making the distinction that the concentration dependent component
of it, k(on), is of less
importance, since in the viscous environment of a cell, the c
4. The physiological concentration is a bit misleading. First, its clear
now that cells have microenvironments,
and 'physiological' concentrations are hard to define. Also, in a cell, I
think (and I think others tend to agree)
that kD plays little role at the end. kD is a combination of k(on) -
Message -
From: "aidong"
To:
Sent: Sunday, July 04, 2010 3:01 AM
Subject: Re: [ccp4bb] monomeric coiled coil--updated
> In light of several wonderful responses,I would like to provide an
> update for this question:
>
> 1. I would agree that SEC might not be able
To:
Sent: Saturday, July 03, 2010 6:26 AM
Subject: [ccp4bb] monomeric coiled coil
Sorry for this ccp4 unrelated question.
We recently have a protein that a multicoil program
(http://groups.csail.mit.edu/cb/multicoil/cgi-bin/multicoil.cgi/cgi-bin/multicoil
) predicts to have very high probabi
quot;
To:
Sent: Saturday, July 03, 2010 6:26 AM
Subject: [ccp4bb] monomeric coiled coil
Sorry for this ccp4 unrelated question.
We recently have a protein that a multicoil program
(http://groups.csail.mit.edu/cb/multicoil/cgi-bin/multicoil.cgi/cgi-bin/multicoil
) predicts to have very high proba
AM
Subject: [ccp4bb] monomeric coiled coil
Sorry for this ccp4 unrelated question.
We recently have a protein that a multicoil program
(http://groups.csail.mit.edu/cb/multicoil/cgi-bin/multicoil.cgi/cgi-bin/multicoil
) predicts to have very high probability for dimer and trimer. Their
sco
Sorry for this ccp4 unrelated question.
We recently have a protein that a multicoil program (http://groups.csail.mit.edu/cb/multicoil/cgi-bin/multicoil.cgi/cgi-bin/multicoil
) predicts to have very high probability for dimer and trimer. Their
scores are close to 0.4 and 0.6 for lengths of mor
