Hi,
To purify your protein you can use GST-Trap HP column, it is quite easy, you
have to elute in a buffer with 20mM reduced glutathione.
HTH,
Catarina
--
Catarina Rodrigues, PhD Student
Molecular Transport & Signalling
AFMB UMR 6098 CNRS/UI/UII Case 932
163 Avenue de Luminy
13288 Marseille
Hi Pascal,
The GST helps a lot with expression, but unfortunately it's very difficult
to purify it away after cleavage. I would strongly recommended to try MBP
tag.
Good luck!
Anna
On Fri, May 27, 2011 at 1:50 PM, Pascal Egea wrote:
>
> Dear All,
>
> This is not strictly a crystallography rel
Dear All,
This is not strictly a crystallography related question.
We are trying to express several membrane proteins and were considering the
use of GST as a fusion partner instead of the HIS or FLAG purification tags.
I would like to know if anyone had any experience (positive or negative) to
sh
